Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 4908388..4909038 Replicon chromosome
Accession NZ_CP026715
Organism Klebsiella oxytoca strain AR_0028

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag AM355_RS23565 Protein ID WP_014227314.1
Coordinates 4908388..4908729 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag AM355_RS23570 Protein ID WP_014227313.1
Coordinates 4908739..4909038 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM355_RS23550 4904651..4905970 + 1320 WP_032751824.1 MFS transporter -
AM355_RS23555 4906116..4907507 + 1392 WP_004107098.1 hexose-6-phosphate:phosphate antiporter -
AM355_RS23560 4907685..4908137 + 453 WP_025107571.1 DUF1198 domain-containing protein -
AM355_RS23565 4908388..4908729 + 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM355_RS23570 4908739..4909038 + 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
AM355_RS23575 4909157..4910338 + 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
AM355_RS23580 4910371..4911180 - 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
AM355_RS23585 4911190..4912293 - 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
AM355_RS23590 4912433..4913152 + 720 WP_085521470.1 phosphonate utilization transcriptional regulator PhnR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4908388..4924432 16044


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T95526 WP_014227314.1 NZ_CP026715:4908388-4908729 [Klebsiella oxytoca]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T95526 NZ_CP026715:4908388-4908729 [Klebsiella oxytoca]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGCTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT95526 WP_014227313.1 NZ_CP026715:4908739-4909038 [Klebsiella oxytoca]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT95526 NZ_CP026715:4908739-4909038 [Klebsiella oxytoca]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AACCCACCATAGCAAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References