Detailed information of TA system TA095500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2166919..2167653
• Replicon: chromosome
• Accession: NZ_CP026709
• Organism: Citrobacter koseri strain AR_0024

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM351_RS10390
• Protein ID: WP_058668984.1
• Coordinates: 2166919..2167224 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A8AI81
• Locus tag: AM351_RS10395
• Protein ID: WP_012132926.1
• Coordinates: 2167312..2167653 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM351_RS10365	2162255..2162974	+	720	WP_058668981.1	aspartate/glutamate racemase family protein	-
AM351_RS10370	2163003..2164010	+	1008	WP_049009338.1	ABC transporter substrate-binding protein	-
AM351_RS10375	2164007..2164777	+	771	WP_058668982.1	ABC transporter permease	-
AM351_RS10380	2164777..2165553	+	777	WP_012132930.1	ABC transporter ATP-binding protein	-
AM351_RS10385	2165587..2166690	-	1104	WP_058668983.1	4Fe-4S binding protein	-
AM351_RS10390	2166919..2167224	+	306	WP_058668984.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM351_RS10395	2167312..2167653	+	342	WP_012132926.1	HigA family addiction module antidote protein	Antitoxin
AM351_RS10400	2167793..2168098	-	306	WP_047457663.1	chaperone modulator CbpM	-
AM351_RS10405	2168098..2169018	-	921	WP_049009332.1	curved DNA-binding protein	-
AM351_RS10410	2169248..2169613	+	366	WP_047457658.1	hypothetical protein	-
AM351_RS10415	2169662..2171548	+	1887	WP_060816117.1	thioredoxin family protein	-
AM351_RS10420	2171545..2172168	+	624	WP_060816116.1	thioredoxin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (10-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11958.68 Da
• Isoelectric Point: 8.3565

>T95500 WP_058668984.1 NZ_CP026709:2166919-2167224 [Citrobacter koseri]
MPKKLVSIEDFRDQWLDDFFMYSAPHKKVPPDIHSALARKLDIINAAVSYKDLKSPPGNRYEELEGKLQEYSSIRVNKQY
RLIFKWVNGKAQDLYLDPHEY

Nucleotide

• Length: 306 bp

>T95500 NZ_CP026709:2166919-2167224 [Citrobacter koseri]
ATGCCAAAGAAACTTGTCAGTATTGAAGACTTCCGTGACCAGTGGCTGGATGATTTTTTTATGTACTCTGCACCACATAA
GAAAGTTCCCCCGGATATACATTCAGCATTGGCCCGTAAGCTCGATATCATAAACGCGGCAGTGAGCTACAAGGATTTGA
AGTCACCGCCGGGTAACAGATACGAAGAACTGGAAGGTAAATTGCAGGAATATTCTTCGATAAGAGTTAACAAGCAATAT
CGTTTGATATTCAAATGGGTGAACGGCAAGGCACAAGACCTCTACCTTGATCCACATGAGTATTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13062.91 Da
• Isoelectric Point: 5.1864

>AT95500 WP_012132926.1 NZ_CP026709:2167312-2167653 [Citrobacter koseri]
MNQATRKPTTVGDILLYEYLEPLDLKINDLAEMLQVHRNTASALVNNNRKLTTDMAFRLSKVFDTSVEFWMNLQTAVDVW
ETENNARLQVEMSRVITASDFLAERQKQEKKVA

Nucleotide

• Length: 342 bp

>AT95500 NZ_CP026709:2167312-2167653 [Citrobacter koseri]
ATGAACCAGGCAACCAGAAAACCCACGACAGTTGGTGATATCCTTCTGTATGAATACCTGGAACCGCTTGACCTCAAAAT
TAATGATCTCGCGGAAATGTTGCAGGTGCATCGTAATACCGCCAGCGCATTGGTTAACAACAATCGTAAACTCACGACTG
ACATGGCTTTTCGCCTGTCTAAAGTTTTCGATACCTCTGTAGAGTTCTGGATGAACCTACAGACTGCTGTAGATGTATGG
GAAACTGAAAACAATGCCCGCCTTCAGGTAGAGATGAGCAGGGTGATTACGGCGAGCGATTTTCTTGCGGAACGTCAGAA
ACAAGAAAAGAAAGTCGCGTGA

Structures

Antitoxin

• AlphaFold DB: A8AI81