Detailed information of TA system TA095470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 35917..36563
• Replicon: plasmid unitig_3_pilon
• Accession: NZ_CP026699
• Organism: Citrobacter koseri strain AR_0025

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: AM352_RS24190
• Protein ID: WP_104869192.1
• Coordinates: 35917..36267 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM352_RS24195
• Protein ID: WP_104869193.1
• Coordinates: 36264..36563 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM352_RS24155	31795..32193	+	399	WP_104869207.1	Exc2 family lipoprotein	-
AM352_RS24165	32932..33213	-	282	WP_104869189.1	hypothetical protein	-
AM352_RS24170	33460..33738	-	279	WP_104869208.1	stability/ partitioning determinant	-
AM352_RS24175	33834..34496	-	663	WP_104869190.1	P-loop NTPase	-
AM352_RS24185	34950..35432	+	483	WP_104869191.1	hypothetical protein	-
AM352_RS24190	35917..36267	+	351	WP_104869192.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM352_RS24195	36264..36563	+	300	WP_104869193.1	XRE family transcriptional regulator	Antitoxin
AM352_RS24205	37176..37481	+	306	WP_104869209.1	hypothetical protein	-
AM352_RS24210	37550..37843	+	294	WP_104869195.1	hypothetical protein	-
AM352_RS24215	37926..38168	+	243	WP_104869196.1	hypothetical protein	-
AM352_RS24220	38158..38373	+	216	WP_104869197.1	hypothetical protein	-
AM352_RS24225	38465..38794	+	330	WP_104869198.1	hypothetical protein	-
AM352_RS24230	38856..39038	+	183	WP_104869199.1	hypothetical protein	-
AM352_RS24245	40148..40525	+	378	WP_104869202.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42930	42930

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13508.44 Da
• Isoelectric Point: 7.2759

>T95470 WP_104869192.1 NZ_CP026699:35917-36267 [Citrobacter koseri]
MWTVYFGRVFDEWFEEQEAGLKQKVLADLSNLEMYGPSLSRPYADTVKGSRHKNMKELRIQYAGHPIRAFFAFDPIRQAI
VLCAGDKSNDKKFYDRMIRIADEEFSAHLAELEGKK

Nucleotide

• Length: 351 bp

>T95470 NZ_CP026699:35917-36267 [Citrobacter koseri]
ATGTGGACGGTTTATTTTGGCAGGGTTTTTGATGAATGGTTTGAGGAGCAGGAAGCTGGCCTGAAACAAAAAGTGCTGGC
TGATCTGAGCAACCTCGAAATGTATGGCCCCTCTCTTTCGCGGCCGTATGCAGATACAGTTAAAGGGTCACGTCATAAGA
ACATGAAGGAACTGCGCATACAGTATGCGGGGCATCCAATCAGGGCATTCTTTGCGTTTGACCCGATTAGACAGGCTATC
GTTCTTTGTGCCGGAGACAAAAGTAACGATAAGAAATTTTACGACAGAATGATTCGTATCGCCGATGAAGAGTTTTCCGC
TCACTTGGCCGAACTGGAGGGCAAAAAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11119.97 Da
• Isoelectric Point: 6.4733

>AT95470 WP_104869193.1 NZ_CP026699:36264-36563 [Citrobacter koseri]
MRTLREVIAAQSPESQARIKEMADEMILETGLQMMREELQLSQKHVAELMGISQPAVTQLEQRGNDVKLATLKRYIEAMG
GKLSLDVELPTGKRVAFHV

Nucleotide

• Length: 300 bp

>AT95470 NZ_CP026699:36264-36563 [Citrobacter koseri]
ATGAGAACATTAAGAGAAGTAATTGCGGCTCAGTCACCTGAAAGTCAGGCTCGTATTAAAGAGATGGCCGATGAAATGAT
TCTGGAGACCGGGCTGCAAATGATGCGTGAAGAGTTGCAGCTTTCGCAGAAACATGTTGCTGAGCTGATGGGGATCAGTC
AACCTGCGGTCACTCAACTGGAGCAGCGCGGAAATGATGTGAAGCTGGCGACTCTGAAACGTTATATCGAGGCGATGGGA
GGAAAGCTAAGCCTTGATGTTGAACTCCCGACTGGTAAGCGTGTGGCGTTCCACGTTTAA