95466

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3624285..3625019 Replicon chromosome
Accession NZ_CP026697
Organism Citrobacter koseri strain AR_0025

Toxin (Protein)


Gene name higB Uniprot ID A8AI82
Locus tag AM352_RS17725 Protein ID WP_012132927.1
Coordinates 3624714..3625019 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A8AI81
Locus tag AM352_RS17720 Protein ID WP_012132926.1
Coordinates 3624285..3624626 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AM352_RS17695 3619770..3620393 - 624 WP_104868814.1 thioredoxin domain-containing protein -
AM352_RS17700 3620390..3622276 - 1887 WP_104868815.1 thioredoxin family protein -
AM352_RS17705 3622325..3622690 - 366 WP_047457658.1 hypothetical protein -
AM352_RS17710 3622920..3623840 + 921 WP_047457661.1 curved DNA-binding protein -
AM352_RS17715 3623840..3624145 + 306 WP_047457663.1 chaperone modulator CbpM -
AM352_RS17720 3624285..3624626 - 342 WP_012132926.1 HigA family addiction module antidote protein Antitoxin
AM352_RS17725 3624714..3625019 - 306 WP_012132927.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AM352_RS17730 3625248..3626354 + 1107 WP_104868816.1 4Fe-4S binding protein -
AM352_RS17735 3626388..3627164 - 777 WP_047457671.1 ABC transporter ATP-binding protein -
AM352_RS17740 3627164..3627934 - 771 WP_058668982.1 ABC transporter permease -
AM352_RS17745 3627931..3628938 - 1008 WP_049009338.1 ABC transporter substrate-binding protein -
AM352_RS17750 3628967..3629686 - 720 WP_104868817.1 aspartate/glutamate racemase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-101)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-69)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11972.70 Da        Isoelectric Point: 8.3565

>T95466 WP_012132927.1 NZ_CP026697:c3625019-3624714 [Citrobacter koseri]
MPKKLVSIEDFRDQWLDDFFMYSAPHKKIPPDIHSALARKLDIINAAVSYKDLKSPPGNRYEELEGKLQEYSSIRVNKQY
RLIFKWVNGKAQDLYLDPHEY

Download         Length: 306 bp

>T95466 NZ_CP026697:c3625019-3624714 [Citrobacter koseri]
ATGCCAAAGAAACTTGTCAGTATTGAAGACTTCCGTGACCAGTGGCTGGATGATTTTTTTATGTACTCTGCACCACATAA
GAAAATTCCCCCGGATATACATTCAGCATTGGCCCGTAAGCTCGATATCATAAACGCGGCAGTGAGCTACAAGGATTTGA
AGTCACCGCCGGGTAACAGATACGAAGAACTGGAAGGTAAATTGCAGGAATATTCTTCGATAAGAGTTAACAAGCAATAT
CGTTTGATATTCAAATGGGTGAACGGCAAGGCACAAGACCTCTACCTTGATCCACATGAGTATTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13062.91 Da        Isoelectric Point: 5.1864

>AT95466 WP_012132926.1 NZ_CP026697:c3624626-3624285 [Citrobacter koseri]
MNQATRKPTTVGDILLYEYLEPLDLKINDLAEMLQVHRNTASALVNNNRKLTTDMAFRLSKVFDTSVEFWMNLQTAVDVW
ETENNARLQVEMSRVITASDFLAERQKQEKKVA

Download         Length: 342 bp

>AT95466 NZ_CP026697:c3624626-3624285 [Citrobacter koseri]
ATGAACCAGGCAACCAGAAAACCCACGACAGTTGGTGATATCCTTCTGTATGAATACCTGGAACCGCTTGATCTCAAAAT
TAATGATCTCGCGGAAATGTTGCAGGTGCATCGTAATACCGCCAGCGCATTGGTTAACAACAATCGTAAACTCACGACTG
ACATGGCTTTTCGCCTGTCTAAAGTTTTCGATACCTCTGTAGAGTTCTGGATGAACCTGCAAACTGCTGTAGATGTATGG
GAAACTGAAAACAATGCCCGCCTTCAGGTAGAGATGAGCAGGGTGATTACGGCGAGCGATTTTCTTGCGGAACGTCAGAA
ACAAGAAAAGAAAGTCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A8AI82

Antitoxin

Source ID Structure
AlphaFold DB A8AI81

References