Detailed information of TA system TA095430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3716632..3717218
• Replicon: chromosome
• Accession: NZ_CP026675
• Organism: Pseudomonas sp. SWI36

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: C4Q27_RS17755
• Protein ID: WP_080574895.1
• Coordinates: 3716940..3717218 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: Q88MI6
• Locus tag: C4Q27_RS17750
• Protein ID: WP_010952669.1
• Coordinates: 3716632..3716931 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4Q27_RS17720	3712478..3713287	+	810	WP_162490563.1	tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA	-
C4Q27_RS17730	3713400..3713810	-	411	WP_104887130.1	SufE family protein	-
C4Q27_RS17735	3713807..3715012	-	1206	WP_104887131.1	cysteine desulfurase	-
C4Q27_RS17740	3715094..3716128	-	1035	WP_019437665.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-
C4Q27_RS17745	3716160..3716507	-	348	WP_003252272.1	ArsC family reductase	-
C4Q27_RS17750	3716632..3716931	-	300	WP_010952669.1	type II toxin-antitoxin system antitoxin GraA	Antitoxin
C4Q27_RS17755	3716940..3717218	-	279	WP_080574895.1	type II toxin-antitoxin system toxin GraT	Toxin
C4Q27_RS17760	3717471..3719117	+	1647	WP_064493150.1	Na+/H+ antiporter	-
C4Q27_RS17770	3719223..3720419	-	1197	WP_104887133.1	succinyldiaminopimelate transaminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10503.82 Da
• Isoelectric Point: 7.8929

>T95430 WP_080574895.1 NZ_CP026675:c3717218-3716940 [Pseudomonas sp. SWI36]
MIRSFSCADTEALFTTGKTRRWSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRAGQHSIRVNDQWRVCFTWTE
HGPVNVEIVDYH

Nucleotide

• Length: 279 bp

>T95430 NZ_CP026675:c3717218-3716940 [Pseudomonas sp. SWI36]
ATGATTCGAAGCTTTAGCTGTGCCGACACGGAAGCGCTCTTCACAACGGGAAAGACACGGCGTTGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAGCTGGCCATGCTCGATGCCGCAACAGAGTTGCGAGACCTTAGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCCGGCCAACACAGTATCCGTGTAAATGACCAATGGCGGGTTTGCTTCACCTGGACAGAA
CACGGGCCAGTTAACGTTGAAATTGTCGATTACCACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10905.55 Da
• Isoelectric Point: 5.6793

>AT95430 WP_010952669.1 NZ_CP026675:c3716931-3716632 [Pseudomonas sp. SWI36]
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Nucleotide

• Length: 300 bp

>AT95430 NZ_CP026675:c3716931-3716632 [Pseudomonas sp. SWI36]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGTGAAATCCTGCGGGAGGAGTTCCAGAAGGAAATGGGCTTCAGCGC
CGCGGCTCTGGCCAGGGCGTTGGGTGTAGCAACGCCAACCGTCAACAACATCCTGCGCGAACGGGGCGGTGTTTCCGCCG
ACATGGCACTGCGCCTGTCCATCTGCCTGGACACCACTCCTGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATCTGCGC
ACAGCCGAACAGCAACACGGCGATGAGATCATCGGGTCGGTCCAGCGTCTGGTCGCTTGA

Structures

Antitoxin

• AlphaFold DB: Q88MI6