Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3716632..3717218 Replicon chromosome
Accession NZ_CP026675
Organism Pseudomonas sp. SWI36

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag C4Q27_RS17755 Protein ID WP_080574895.1
Coordinates 3716940..3717218 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88MI6
Locus tag C4Q27_RS17750 Protein ID WP_010952669.1
Coordinates 3716632..3716931 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4Q27_RS17720 3712478..3713287 + 810 WP_162490563.1 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA -
C4Q27_RS17730 3713400..3713810 - 411 WP_104887130.1 SufE family protein -
C4Q27_RS17735 3713807..3715012 - 1206 WP_104887131.1 cysteine desulfurase -
C4Q27_RS17740 3715094..3716128 - 1035 WP_019437665.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
C4Q27_RS17745 3716160..3716507 - 348 WP_003252272.1 ArsC family reductase -
C4Q27_RS17750 3716632..3716931 - 300 WP_010952669.1 type II toxin-antitoxin system antitoxin GraA Antitoxin
C4Q27_RS17755 3716940..3717218 - 279 WP_080574895.1 type II toxin-antitoxin system toxin GraT Toxin
C4Q27_RS17760 3717471..3719117 + 1647 WP_064493150.1 Na+/H+ antiporter -
C4Q27_RS17770 3719223..3720419 - 1197 WP_104887133.1 succinyldiaminopimelate transaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10503.82 Da        Isoelectric Point: 7.8929

>T95430 WP_080574895.1 NZ_CP026675:c3717218-3716940 [Pseudomonas sp. SWI36]
MIRSFSCADTEALFTTGKTRRWSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRAGQHSIRVNDQWRVCFTWTE
HGPVNVEIVDYH

Download         Length: 279 bp

>T95430 NZ_CP026675:c3717218-3716940 [Pseudomonas sp. SWI36]
ATGATTCGAAGCTTTAGCTGTGCCGACACGGAAGCGCTCTTCACAACGGGAAAGACACGGCGTTGGTCAGATATCAAGTC
AGTCGCGGAGCGCAAGCTGGCCATGCTCGATGCCGCAACAGAGTTGCGAGACCTTAGGTCGCCACCTGGCAATCGCCTCG
AGTCACTGAGCGGCAACCGCGCCGGCCAACACAGTATCCGTGTAAATGACCAATGGCGGGTTTGCTTCACCTGGACAGAA
CACGGGCCAGTTAACGTTGAAATTGTCGATTACCACTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10905.55 Da        Isoelectric Point: 5.6793

>AT95430 WP_010952669.1 NZ_CP026675:c3716931-3716632 [Pseudomonas sp. SWI36]
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Download         Length: 300 bp

>AT95430 NZ_CP026675:c3716931-3716632 [Pseudomonas sp. SWI36]
ATGCTCAAGAACGGTATGCGTCCAATCCATCCGGGTGAAATCCTGCGGGAGGAGTTCCAGAAGGAAATGGGCTTCAGCGC
CGCGGCTCTGGCCAGGGCGTTGGGTGTAGCAACGCCAACCGTCAACAACATCCTGCGCGAACGGGGCGGTGTTTCCGCCG
ACATGGCACTGCGCCTGTCCATCTGCCTGGACACCACTCCTGAGTTCTGGCTCAACCTGCAGACGGCCTTCGATCTGCGC
ACAGCCGAACAGCAACACGGCGATGAGATCATCGGGTCGGTCCAGCGTCTGGTCGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6F8S
AlphaFold DB Q88MI6

References