Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3701494..3702035 Replicon chromosome
Accession NZ_CP026641
Organism Escherichia coli strain FORC_082

Toxin (Protein)


Gene name yafQ Uniprot ID A0A140NAP5
Locus tag FORC82_RS18415 Protein ID WP_000615981.1
Coordinates 3701757..3702035 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FORC82_RS18410 Protein ID WP_000729704.1
Coordinates 3701494..3701754 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FORC82_RS18390 (3697176) 3697176..3697961 - 786 WP_000207564.1 putative lateral flagellar export/assembly protein LafU -
FORC82_RS18395 (3697933) 3697933..3699646 + 1714 Protein_3588 flagellar biosynthesis protein FlhA -
FORC82_RS18400 (3699862) 3699862..3700359 - 498 WP_000006260.1 REP-associated tyrosine transposase RayT -
FORC82_RS18405 (3700535) 3700535..3701308 - 774 WP_001353765.1 C40 family peptidase -
FORC82_RS18410 (3701494) 3701494..3701754 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FORC82_RS18415 (3701757) 3701757..3702035 + 279 WP_000615981.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FORC82_RS18420 (3702191) 3702191..3702931 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
FORC82_RS18425 (3702902) 3702902..3703669 - 768 WP_000333379.1 class II glutamine amidotransferase -
FORC82_RS18430 (3703875) 3703875..3704453 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10860.65 Da        Isoelectric Point: 10.0702

>T95359 WP_000615981.1 NZ_CP026641:3701757-3702035 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQSSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T95359 NZ_CP026641:3701757-3702035 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT95359 WP_000729704.1 NZ_CP026641:3701494-3701754 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT95359 NZ_CP026641:3701494-3701754 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MMG
PDB 4MMJ
PDB 4ML2
PDB 4ML0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References