Detailed information of TA system TA095306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 15462..16047
• Replicon: plasmid pSWBY1
• Accession: NZ_CP026617
• Organism: Acinetobacter sp. SWBY1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9BBX7
• Locus tag: C3Y93_RS14135
• Protein ID: WP_004977497.1
• Coordinates: 15727..16047 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C3Y93_RS14130
• Protein ID: WP_000369782.1
• Coordinates: 15462..15734 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3Y93_RS14110	10569..10934	+	366	WP_004201167.1	bleomycin binding protein Ble-MBL	-
C3Y93_RS14115	10939..11166	+	228	Protein_14	N-(5'-phosphoribosyl)anthranilate isomerase	-
C3Y93_RS14120	11539..12186	-	648	WP_104853307.1	recombinase family protein	-
C3Y93_RS14125	12995..13920	+	926	Protein_16	IS5 family transposase	-
C3Y93_RS14130	15462..15734	-	273	WP_000369782.1	helix-turn-helix domain-containing protein	Antitoxin
C3Y93_RS14135	15727..16047	-	321	WP_004977497.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3Y93_RS14140	16206..18734	-	2529	WP_104853308.1	relaxase/mobilization nuclease domain-containing protein	-
C3Y93_RS14145	18718..19260	-	543	WP_104853309.1	MobC family plasmid mobilization relaxosome protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / aph(3')-VI / blaNDM-1	-	1..38161	38161
	-	inside	IScluster/Tn	mph(E) / msr(E) / aph(3')-VI / blaNDM-1	-	1327..13920	12593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12265.08 Da
• Isoelectric Point: 7.9849

>T95306 WP_004977497.1 NZ_CP026617:c16047-15727 [Acinetobacter sp. SWBY1]
MLFIETSIFTKQIRELVTDEEYRQLQQDLLIQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T95306 NZ_CP026617:c16047-15727 [Acinetobacter sp. SWBY1]
ATGTTATTCATTGAAACTAGTATCTTTACCAAGCAAATTAGAGAGCTTGTCACTGATGAAGAATATCGTCAGCTCCAGCA
AGATCTCCTGATACAGCCTGATAAGGGTGACTTAATCAAGAATGGTGGAGGAATTCGTAAAGTACGTTGTGCTCAAGGCA
ATAAAGGAAAAAGTGGTGGTATCCGCGTGATCTATTATTGGGTCACAGAAGATGATCAGATCTTTTTTCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGATAAAGAAACCTCGATCCTGCGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10064.50 Da
• Isoelectric Point: 7.2045

>AT95306 WP_000369782.1 NZ_CP026617:c15734-15462 [Acinetobacter sp. SWBY1]
MDNNLFDDLVASIKEAGAIKRNEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPGLI

Nucleotide

• Length: 273 bp

>AT95306 NZ_CP026617:c15734-15462 [Acinetobacter sp. SWBY1]
ATGGATAACAACTTATTTGATGATCTGGTTGCTTCAATCAAAGAAGCTGGGGCCATCAAACGTAATGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAACTGCCAGATATTAAGGAAGTACGTGAAAAAACAGGCTTAACCCAAGCTGAATTTGCTGCAC
GTTTGCATATCAGCGCGAGAACCCTGCAAAACTGGGAACAAGGCCGGCGTTATCCGACTGGCCCTGCAGCGACGTTAATC
CGTATTCTGGATGCTCACCCGGGTCTGATTTAA

Structures

Toxin

• AlphaFold DB: N9BBX7