Detailed information of TA system TA095304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4235..4820
• Replicon: plasmid pSWBY1
• Accession: NZ_CP026617
• Organism: Acinetobacter sp. SWBY1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: C3Y93_RS14070
• Protein ID: WP_000897307.1
• Coordinates: 4500..4820 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C3Y93_RS14065
• Protein ID: WP_000369781.1
• Coordinates: 4235..4507 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3Y93_RS14050	526..1137	+	612	WP_044432563.1	recombinase family protein	-
C3Y93_RS14055	1327..2211	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
C3Y93_RS14060	2267..3742	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C3Y93_RS14065	4235..4507	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
C3Y93_RS14070	4500..4820	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3Y93_RS14075	4960..5379	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
C3Y93_RS14080	5464..5745	-	282	WP_000985609.1	putative addiction module antidote protein	-
C3Y93_RS14085	5746..6048	-	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C3Y93_RS14090	6377..7521	+	1145	WP_104853306.1	IS3 family transposase	-
C3Y93_RS14095	7696..8475	+	780	WP_075594633.1	APH(3')-VI family aminoglycoside O-phosphotransferase	-
C3Y93_RS14100	8627..9652	+	1026	WP_004994718.1	IS30-like element ISAba125 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / aph(3')-VI / blaNDM-1	-	1..38161	38161
	-	inside	IScluster/Tn	mph(E) / msr(E) / aph(3')-VI / blaNDM-1	-	1327..13920	12593

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T95304 WP_000897307.1 NZ_CP026617:c4820-4500 [Acinetobacter sp. SWBY1]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T95304 NZ_CP026617:c4820-4500 [Acinetobacter sp. SWBY1]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT95304 WP_000369781.1 NZ_CP026617:c4507-4235 [Acinetobacter sp. SWBY1]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT95304 NZ_CP026617:c4507-4235 [Acinetobacter sp. SWBY1]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3