Detailed information of TA system TA095300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1863903..1864554
• Replicon: chromosome
• Accession: NZ_CP026616
• Organism: Acinetobacter sp. SWBY1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9NM16
• Locus tag: C3Y93_RS09045
• Protein ID: WP_004668307.1
• Coordinates: 1864198..1864554 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C3Y93_RS09040
• Protein ID: WP_005237493.1
• Coordinates: 1863903..1864205 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3Y93_RS09005	1859268..1859792	-	525	WP_104472915.1	TIGR00645 family protein	-
C3Y93_RS09010	1859786..1859946	-	161	Protein_1745	DUF475 domain-containing protein	-
C3Y93_RS09015	1860054..1861028	+	975	WP_005103529.1	calcium/sodium antiporter	-
C3Y93_RS09020	1861096..1861800	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
C3Y93_RS09025	1861864..1863144	-	1281	WP_005105969.1	cation transporter	-
C3Y93_RS09030	1863235..1863627	+	393	WP_104852708.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
C3Y93_RS09035	1863700..1863861	+	162	Protein_1750	IS6 family transposase	-
C3Y93_RS09040	1863903..1864205	-	303	WP_005237493.1	helix-turn-helix transcriptional regulator	Antitoxin
C3Y93_RS09045	1864198..1864554	-	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3Y93_RS09050	1865335..1865664	+	330	WP_004827037.1	four-helix bundle copper-binding protein	-
C3Y93_RS09055	1865883..1867034	+	1152	WP_058951619.1	IS4 family transposase	-
C3Y93_RS09060	1867072..1867365	-	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C3Y93_RS09065	1867355..1867600	-	246	WP_000246755.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
C3Y93_RS09070	1867780..1868724	-	945	WP_104852709.1	copper resistance protein B	-
C3Y93_RS09075	1868711..1869049	-	339	WP_004897649.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	tet(39) / blaOXA-58 / aph(3'')-Ib / aph(6)-Id / sul2 / floR	-	1819901..1867034	47133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.64 Da
• Isoelectric Point: 7.1651

>T95300 WP_004668307.1 NZ_CP026616:c1864554-1864198 [Acinetobacter sp. SWBY1]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T95300 NZ_CP026616:c1864554-1864198 [Acinetobacter sp. SWBY1]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAACCTACACAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCGTCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11208.99 Da
• Isoelectric Point: 6.4759

>AT95300 WP_005237493.1 NZ_CP026616:c1864205-1863903 [Acinetobacter sp. SWBY1]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTLKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT95300 NZ_CP026616:c1864205-1863903 [Acinetobacter sp. SWBY1]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCTTGAAAAAGTATGTTGAGGCAATG
GGAGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA

Structures

Toxin

• AlphaFold DB: N9NM16