Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3615207..3615709 Replicon chromosome
Accession NZ_CP026612
Organism Escherichia coli strain DTU-1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C4J63_RS18735 Protein ID WP_000767829.1
Coordinates 3615207..3615461 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C4J63_RS18740 Protein ID WP_001259255.1
Coordinates 3615458..3615709 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4J63_RS18705 3610222..3610449 - 228 WP_000234896.1 sulfurtransferase TusA -
C4J63_RS18710 3610463..3611521 - 1059 WP_000492339.1 transport protein YeeE -
C4J63_RS18715 3611700..3613058 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C4J63_RS18720 3613048..3613110 - 63 WP_010723108.1 membrane protein YoeI -
C4J63_RS18725 3613325..3614254 - 930 WP_000803351.1 LysR family transcriptional regulator -
C4J63_RS18730 3614300..3615124 - 825 WP_000754737.1 SDR family oxidoreductase -
C4J63_RS18735 3615207..3615461 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C4J63_RS18740 3615458..3615709 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C4J63_RS18745 3615992..3616042 + 51 WP_001364200.1 his operon leader peptide -
C4J63_RS18755 3616188..3617087 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C4J63_RS18760 3617093..3618397 + 1305 WP_000009594.1 histidinol dehydrogenase -
C4J63_RS18765 3618394..3619464 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
C4J63_RS18770 3619464..3620531 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T95285 WP_000767829.1 NZ_CP026612:c3615461-3615207 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T95285 NZ_CP026612:c3615461-3615207 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT95285 WP_001259255.1 NZ_CP026612:c3615709-3615458 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT95285 NZ_CP026612:c3615709-3615458 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References