Detailed information of TA system TA095203

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37690..37959
• Replicon: plasmid p3
• Accession: NZ_CP026589
• Organism: Klebsiella pneumoniae strain NUHL30457

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C3483_RS30855
• Protein ID: WP_001312861.1
• Coordinates: 37801..37959 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37690..37755 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3483_RS28895	33400..33927	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
C3483_RS28900	33985..34218	+	234	WP_000006003.1	DUF905 family protein	-
C3483_RS28905	34279..36302	+	2024	Protein_46	ParB/RepB/Spo0J family partition protein	-
C3483_RS28910	36371..36805	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C3483_RS28915	36802..37521	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	37533..37757	+	225	NuclAT_0	-	-
-	37533..37757	+	225	NuclAT_0	-	-
-	37533..37757	+	225	NuclAT_0	-	-
-	37533..37757	+	225	NuclAT_0	-	-
-	37690..37755	+	66	-	-	Antitoxin
C3483_RS30855	37801..37959	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
C3483_RS31005	38197..38574	-	378	Protein_50	hypothetical protein	-
C3483_RS28940	38874..39170	+	297	WP_001272251.1	hypothetical protein	-
C3483_RS28945	39281..40102	+	822	WP_001234445.1	DUF945 domain-containing protein	-
C3483_RS28950	40399..41046	-	648	WP_104716617.1	transglycosylase SLT domain-containing protein	-
C3483_RS28955	41323..41706	+	384	WP_000124981.1	relaxosome protein TraM	-
C3483_RS28960	41897..42583	+	687	WP_015059009.1	PAS domain-containing protein	-
C3483_RS28965	42677..42904	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2	-	1..89247	89247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T95203 WP_001312861.1 NZ_CP026589:37801-37959 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T95203 NZ_CP026589:37801-37959 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT95203 NZ_CP026589:37690-37755 [Klebsiella pneumoniae]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin