Detailed information of TA system TA095159

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 155003..155256
• Replicon: plasmid pKPC2_095649
• Accession: NZ_CP026584
• Organism: Klebsiella pneumoniae strain WCHKP649

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: BT120_RS01265
• Protein ID: WP_001312851.1
• Coordinates: 155107..155256 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	155003..155062 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BT120_RS01220	150663..150728	-	66	Protein_219	hypothetical protein	-
BT120_RS01225	150781..151485	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
BT120_RS01230	151549..151710	+	162	Protein_221	DNA helicase	-
BT120_RS01235	151730..152476	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
BT120_RS01240	152531..153091	+	561	WP_000139328.1	fertility inhibition protein FinO	-
BT120_RS01245	153223..153423	+	201	WP_015059022.1	hypothetical protein	-
BT120_RS01250	153809..154408	+	600	WP_032083981.1	hypothetical protein	-
BT120_RS01255	154572..154802	+	231	WP_001736714.1	hypothetical protein	-
BT120_RS01260	154825..155052	-	228	Protein_227	hypothetical protein	-
-	155003..155062	-	60	NuclAT_1	-	Antitoxin
-	155003..155062	-	60	NuclAT_1	-	Antitoxin
-	155003..155062	-	60	NuclAT_1	-	Antitoxin
-	155003..155062	-	60	NuclAT_1	-	Antitoxin
BT120_RS01265	155107..155256	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
BT120_RS01270	155540..155788	+	249	WP_000083837.1	replication regulatory protein RepA	-
BT120_RS01275	155789..155998	-	210	WP_064765370.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB / catA2	-	1..156099	156099

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T95159 WP_001312851.1 NZ_CP026584:155107-155256 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T95159 NZ_CP026584:155107-155256 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT95159 NZ_CP026584:c155062-155003 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin