Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3753976..3754670 Replicon chromosome
Accession NZ_CP026580
Organism Escherichia coli strain WCHEC005237

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag C4E05_RS20490 Protein ID WP_001263489.1
Coordinates 3753976..3754374 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag C4E05_RS20495 Protein ID WP_000554758.1
Coordinates 3754377..3754670 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3749564..3749644 - 81 NuclAT_11 - -
- 3749564..3749644 - 81 NuclAT_11 - -
- 3749564..3749644 - 81 NuclAT_11 - -
- 3749564..3749644 - 81 NuclAT_11 - -
C4E05_RS20465 3750240..3750698 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
C4E05_RS20470 3750959..3752416 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
C4E05_RS20475 3752473..3752994 - 522 Protein_3513 peptide chain release factor H -
C4E05_RS20480 3752990..3753196 - 207 Protein_3514 RtcB family protein -
C4E05_RS20485 3753514..3753966 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
C4E05_RS20490 3753976..3754374 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
C4E05_RS20495 3754377..3754670 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
C4E05_RS20500 3754722..3755777 - 1056 WP_001226164.1 DNA polymerase IV -
C4E05_RS20505 3755848..3756633 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
C4E05_RS20510 3756578..3758317 + 1740 WP_001334802.1 flagellar biosynthesis protein FlhA -
C4E05_RS20515 3758541..3759038 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T95147 WP_001263489.1 NZ_CP026580:c3754374-3753976 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T95147 NZ_CP026580:c3754374-3753976 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT95147 WP_000554758.1 NZ_CP026580:c3754670-3754377 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT95147 NZ_CP026580:c3754670-3754377 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References