Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1856665..1857167 Replicon chromosome
Accession NZ_CP026580
Organism Escherichia coli strain WCHEC005237

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C4E05_RS11025 Protein ID WP_000767829.1
Coordinates 1856913..1857167 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C4E05_RS11020 Protein ID WP_001259255.1
Coordinates 1856665..1856916 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C4E05_RS10995 1851842..1852909 - 1068 WP_046464156.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C4E05_RS11000 1852909..1853979 - 1071 WP_000108945.1 histidinol-phosphate transaminase -
C4E05_RS11005 1853976..1855280 - 1305 WP_001446927.1 histidinol dehydrogenase -
C4E05_RS11010 1855286..1856185 - 900 WP_032329340.1 ATP phosphoribosyltransferase -
C4E05_RS11015 1856331..1856381 - 51 WP_001364200.1 his operon leader peptide -
C4E05_RS11020 1856665..1856916 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C4E05_RS11025 1856913..1857167 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C4E05_RS11030 1857250..1858074 + 825 WP_000754749.1 SDR family oxidoreductase -
C4E05_RS11035 1858120..1859049 + 930 WP_000803351.1 LysR family transcriptional regulator -
C4E05_RS11040 1859264..1859326 + 63 WP_010723108.1 membrane protein YoeI -
C4E05_RS11045 1859316..1860674 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C4E05_RS11050 1860853..1861911 + 1059 WP_000492321.1 transport protein YeeE -
C4E05_RS11055 1861925..1862152 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T95133 WP_000767829.1 NZ_CP026580:1856913-1857167 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T95133 NZ_CP026580:1856913-1857167 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT95133 WP_001259255.1 NZ_CP026580:1856665-1856916 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT95133 NZ_CP026580:1856665-1856916 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References