Detailed information of TA system TA095122

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 66615..67041
• Replicon: plasmid pRmtB1_005237
• Accession: NZ_CP026579
• Organism: Escherichia coli strain WCHEC005237

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: C4E05_RS01945
• Protein ID: WP_034169443.1
• Coordinates: 66615..66773 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	66817..67041 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4E05_RS01900	61665..61892	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
C4E05_RS01905	61986..62672	-	687	WP_001825184.1	PAS domain-containing protein	-
C4E05_RS01910	62863..63246	-	384	WP_000124981.1	relaxosome protein TraM	-
C4E05_RS01915	63523..64170	+	648	WP_000614935.1	transglycosylase SLT domain-containing protein	-
C4E05_RS01920	64467..65288	-	822	WP_001234469.1	DUF945 domain-containing protein	-
C4E05_RS01925	65406..65693	-	288	WP_000107535.1	hypothetical protein	-
C4E05_RS01930	65718..65924	-	207	WP_000547971.1	hypothetical protein	-
C4E05_RS01945	66615..66773	-	159	WP_034169443.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	66817..67041	-	225	NuclAT_0	-	Antitoxin
-	66817..67041	-	225	NuclAT_0	-	Antitoxin
-	66817..67041	-	225	NuclAT_0	-	Antitoxin
-	66817..67041	-	225	NuclAT_0	-	Antitoxin
C4E05_RS25715	66853..67041	+	189	WP_001299721.1	hypothetical protein	-
C4E05_RS01950	67053..67772	-	720	WP_050858785.1	plasmid SOS inhibition protein A	-
C4E05_RS01955	67769..68203	-	435	WP_032217500.1	conjugation system SOS inhibitor PsiB	-
C4E05_RS01960	68258..70216	-	1959	WP_072652246.1	ParB/RepB/Spo0J family partition protein	-
C4E05_RS01965	70281..70514	-	234	WP_000006030.1	DUF905 family protein	-
C4E05_RS01970	70576..71115	-	540	WP_009426937.1	single-stranded DNA-binding protein	-
C4E05_RS01975	71141..71347	-	207	WP_000547971.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / rmtB / sul1 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(A)	-	1..100229	100229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T95122 WP_034169443.1 NZ_CP026579:c66773-66615 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCELRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T95122 NZ_CP026579:c66773-66615 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGCTTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT95122 NZ_CP026579:c67041-66817 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin