Detailed information of TA system TA095119

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69592..69845
• Replicon: plasmid pCTX-M-55_005237
• Accession: NZ_CP026576
• Organism: Escherichia coli strain WCHEC005237

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: C4E05_RS00615
• Protein ID: WP_001312851.1
• Coordinates: 69696..69845 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	69592..69651 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4E05_RS00585	66319..67065	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
C4E05_RS00590	67120..67680	+	561	WP_000139328.1	fertility inhibition protein FinO	-
C4E05_RS00595	67812..68012	+	201	WP_015059022.1	hypothetical protein	-
C4E05_RS00600	68398..68997	+	600	WP_032083981.1	hypothetical protein	-
C4E05_RS00605	69161..69391	+	231	WP_001736714.1	hypothetical protein	-
-	69592..69651	-	60	NuclAT_1	-	Antitoxin
-	69592..69651	-	60	NuclAT_1	-	Antitoxin
-	69592..69651	-	60	NuclAT_1	-	Antitoxin
-	69592..69651	-	60	NuclAT_1	-	Antitoxin
C4E05_RS00615	69696..69845	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
C4E05_RS00620	70129..70377	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / blaTEM-1B / blaCTX-M-55	-	1..70688	70688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T95119 WP_001312851.1 NZ_CP026576:69696-69845 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T95119 NZ_CP026576:69696-69845 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT95119 NZ_CP026576:c69651-69592 [Escherichia coli]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin