Detailed information of TA system TA095115

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30050..30320
• Replicon: plasmid pCTX-M-55_005237
• Accession: NZ_CP026576
• Organism: Escherichia coli strain WCHEC005237

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C4E05_RS00370
• Protein ID: WP_001312861.1
• Coordinates: 30162..30320 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30050..30113 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C4E05_RS00345	25761..26288	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
C4E05_RS00350	26346..26579	+	234	WP_000006003.1	DUF905 family protein	-
C4E05_RS00355	26640..28663	+	2024	Protein_37	ParB/RepB/Spo0J family partition protein	-
C4E05_RS00360	28732..29166	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C4E05_RS00365	29163..29882	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	29894..30118	+	225	NuclAT_0	-	-
-	29894..30118	+	225	NuclAT_0	-	-
-	29894..30118	+	225	NuclAT_0	-	-
-	29894..30118	+	225	NuclAT_0	-	-
C4E05_RS25665	29903..30082	-	180	WP_001309233.1	hypothetical protein	-
-	30050..30113	-	64	-	-	Antitoxin
C4E05_RS00370	30162..30320	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
C4E05_RS25670	30558..30935	-	378	Protein_42	hypothetical protein	-
C4E05_RS00390	31235..31531	+	297	WP_001272251.1	hypothetical protein	-
C4E05_RS00395	31642..32463	+	822	WP_001234445.1	DUF945 domain-containing protein	-
C4E05_RS00400	32760..33407	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
C4E05_RS00405	33684..34067	+	384	WP_000124981.1	relaxosome protein TraM	-
C4E05_RS00410	34258..34944	+	687	WP_015059009.1	PAS domain-containing protein	-
C4E05_RS00415	35038..35265	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	fosA3 / blaTEM-1B / blaCTX-M-55	-	1..70688	70688

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T95115 WP_001312861.1 NZ_CP026576:30162-30320 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T95115 NZ_CP026576:30162-30320 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT95115 NZ_CP026576:c30113-30050 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin