Detailed information of TA system TA095102

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 216029..216622
• Replicon: chromosome
• Accession: NZ_CP026562
• Organism: Pseudomonas avellanae strain R2leaf

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: BKM03_RS01155
• Protein ID: WP_005739894.1
• Coordinates: 216029..216307 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: Q88B19
• Locus tag: BKM03_RS01160
• Protein ID: WP_005739893.1
• Coordinates: 216317..216622 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BKM03_RS01135	211182..212015	-	834	WP_005739898.1	transporter substrate-binding domain-containing protein	-
BKM03_RS01140	212043..212915	-	873	WP_005613618.1	phytanoyl-CoA dioxygenase family protein	-
BKM03_RS01145	212940..214460	-	1521	WP_005739897.1	amino acid ABC transporter permease/ATP-binding protein	-
BKM03_RS01150	214945..215858	+	914	Protein_203	LysR family transcriptional regulator	-
BKM03_RS01155	216029..216307	+	279	WP_005739894.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BKM03_RS01160	216317..216622	+	306	WP_005739893.1	HigA family addiction module antidote protein	Antitoxin
BKM03_RS01165	216656..216937	-	282	WP_005739892.1	accessory factor UbiK family protein	-
BKM03_RS01175	217357..217695	+	339	WP_002555808.1	P-II family nitrogen regulator	-
BKM03_RS01180	217730..219067	+	1338	WP_005739890.1	ammonium transporter	-
BKM03_RS01190	219310..219735	+	426	WP_005739889.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
BKM03_RS01195	219834..220166	+	333	WP_003377934.1	hypothetical protein	-
BKM03_RS01200	220241..220933	-	693	WP_005739888.1	HAD family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10411.07 Da
• Isoelectric Point: 9.1490

>T95102 WP_005739894.1 NZ_CP026562:216029-216307 [Pseudomonas avellanae]
MIVSFKCVHTRYLFLQGKTRLLPSIKSVAERKLAMLDAATSILDLRSPPGNRLEALDGSRSGQYSVRINAQFRICFVWSI
NGPEDVEIVDYH

Nucleotide

• Length: 279 bp

>T95102 NZ_CP026562:216029-216307 [Pseudomonas avellanae]
ATGATCGTGAGTTTTAAGTGCGTGCACACGAGATATCTCTTTTTACAAGGCAAGACGCGGTTGTTGCCATCGATTAAATC
TGTTGCCGAGCGCAAGCTCGCAATGCTTGATGCTGCTACCTCGATTTTGGATCTGCGCTCTCCTCCCGGCAATCGACTAG
AGGCGCTTGATGGAAGTCGTTCTGGCCAATACAGCGTACGTATAAACGCTCAATTCAGGATTTGCTTCGTTTGGAGCATC
AACGGTCCCGAGGACGTAGAAATTGTCGATTATCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11158.01 Da
• Isoelectric Point: 7.7509

>AT95102 WP_005739893.1 NZ_CP026562:216317-216622 [Pseudomonas avellanae]
MNKNGMRPVHPGEVLKEEYLEPMGLTAAALARALKVSTPTVNDIVLQRRGVSADVALRLAVCLETSPEFWLNLQLAYDLR
KAETEKGAQIREQVKCLAHCA

Nucleotide

• Length: 306 bp

>AT95102 NZ_CP026562:216317-216622 [Pseudomonas avellanae]
ATGAACAAAAACGGAATGCGCCCCGTCCATCCAGGCGAGGTTCTGAAAGAGGAATATCTTGAGCCGATGGGGCTGACCGC
CGCAGCGTTGGCCAGGGCCCTTAAGGTTTCTACGCCGACGGTGAACGACATCGTGCTCCAGCGCAGAGGTGTGAGCGCTG
ATGTAGCGCTGCGTCTGGCTGTATGCCTGGAGACGAGTCCTGAGTTCTGGCTTAACCTGCAGCTTGCCTACGATCTGCGC
AAGGCAGAGACAGAGAAGGGCGCGCAGATTCGTGAGCAGGTCAAATGTCTGGCCCACTGCGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0K8LYY6