Detailed information of TA system TA095078

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrH-as-bsrG/-
• Location: 3857589..3857823
• Replicon: chromosome
• Accession: NZ_CP026533
• Organism: Bacillus velezensis strain DKU_NT_04

Toxin (Protein)

• Gene name: bsrH
• Uniprot ID: -
• Locus tag: C3438_RS19975
• Protein ID: WP_104843506.1
• Coordinates: 3857686..3857823 (-)
• Length: 46 a.a.

Antitoxin (RNA)

Gene name	as-bsrG
Locus tag	-
Coordinates	3857589..3857739 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3438_RS19950	3852918..3853901	-	984	WP_065180788.1	thermonuclease family protein	-
C3438_RS19955	3854157..3854594	+	438	WP_076983291.1	SMI1/KNR4 family protein	-
C3438_RS19960	3854631..3856517	+	1887	WP_104843504.1	HNH endonuclease	-
C3438_RS19965	3856530..3856988	+	459	WP_104843505.1	SMI1/KNR4 family protein	-
C3438_RS19970	3857014..3857499	+	486	WP_065180785.1	SMI1/KNR4 family protein	-
-	3857589..3857739	+	151	NuclAT_1	-	Antitoxin
-	3857589..3857739	+	151	NuclAT_1	-	Antitoxin
-	3857589..3857739	+	151	NuclAT_1	-	Antitoxin
-	3857589..3857739	+	151	NuclAT_1	-	Antitoxin
C3438_RS19975	3857686..3857823	-	138	WP_104843506.1	putative holin-like toxin	Toxin
C3438_RS19980	3858048..3858515	+	468	WP_063636675.1	DUF4879 domain-containing protein	-
C3438_RS19985	3858521..3858880	+	360	WP_021493945.1	hypothetical protein	-
C3438_RS19990	3858908..3860155	-	1248	WP_031600262.1	IS256-like element ISBsu2 family transposase	-
C3438_RS20000	3860319..3861338	-	1020	WP_065180795.1	hypothetical protein	-
C3438_RS20005	3861405..3862097	-	693	WP_065180794.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3854109..3866501	12392
	-	flank	IS/Tn	-	-	3858908..3860146	1238

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (3-29)

Sequences

Toxin

Protein

• Length: 46 a.a.
• Molecular weight: 5096.15 Da
• Isoelectric Point: 8.6040

>T95078 WP_104843506.1 NZ_CP026533:c3857823-3857686 [Bacillus velezensis]
MLMSTFQALMLMLAFGSFIIALLTYINKKQTSLETGILKGQVYHE

Nucleotide

• Length: 138 bp

>T95078 NZ_CP026533:c3857823-3857686 [Bacillus velezensis]
ATGCTTATGTCAACATTTCAGGCATTAATGTTAATGCTTGCATTTGGGTCGTTTATAATCGCCCTGTTGACGTATATAAA
TAAAAAACAGACCTCCCTTGAGACTGGAATCCTGAAGGGACAGGTCTATCACGAATAA

Antitoxin

Nucleotide

• Length: 151 bp

>AT95078 NZ_CP026533:3857589-3857739 [Bacillus velezensis]
TTTCTTTAATAAGAAATGCATAAAATAAAAAGACCAGGGTGTTGGTAGCACCCCGGCTCTGTACAAAAAGCTACCCATCA
AAGGGCTTGCTCAGCAGTTATTCGTGATAGACCTGTCCCTTCAGGATTCCAGTCTCAAGGGAGGTCTGTTT

Structures

Antitoxin