Detailed information of TA system TA095045

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1155055..1155673
• Replicon: chromosome
• Accession: NZ_CP026525
• Organism: Sinorhizobium meliloti strain AK21

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q92R61
• Locus tag: C3L21_RS05470
• Protein ID: WP_010969011.1
• Coordinates: 1155377..1155673 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C3L21_RS05465
• Protein ID: WP_010969010.1
• Coordinates: 1155055..1155363 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3L21_RS05420	1150134..1150454	-	321	WP_003527320.1	YnfA family protein	-
C3L21_RS05425	1150529..1150702	-	174	WP_010969006.1	hypothetical protein	-
C3L21_RS05430	1150716..1150889	-	174	WP_003527318.1	hypothetical protein	-
C3L21_RS05435	1151125..1151826	+	702	WP_014529127.1	glutathione S-transferase N-terminal domain-containing protein	-
C3L21_RS05440	1152050..1152391	-	342	WP_010969008.1	DUF2147 domain-containing protein	-
C3L21_RS05445	1152976..1153242	+	267	WP_003527312.1	sel1 repeat family protein	-
C3L21_RS05450	1153264..1154385	-	1122	WP_010969009.1	pyridoxal phosphate-dependent aminotransferase	-
C3L21_RS05460	1154814..1155065	-	252	WP_042708639.1	hypothetical protein	-
C3L21_RS05465	1155055..1155363	-	309	WP_010969010.1	HigA family addiction module antidote protein	Antitoxin
C3L21_RS05470	1155377..1155673	-	297	WP_010969011.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3L21_RS05475	1155721..1156026	-	306	WP_003527305.1	hypothetical protein	-
C3L21_RS05480	1156354..1157391	+	1038	WP_003527304.1	porin	-
C3L21_RS05485	1157501..1158568	-	1068	WP_164823154.1	site-specific integrase	-
C3L21_RS05490	1158609..1159658	-	1050	WP_013844229.1	porin	-
C3L21_RS05495	1159872..1160360	-	489	WP_013844230.1	BA14K family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11677.11 Da
• Isoelectric Point: 6.7260

>T95045 WP_010969011.1 NZ_CP026525:c1155673-1155377 [Sinorhizobium meliloti]
VIVGFRDDWLRTFFVDDVRSRNIPYDLEARLFRKLQMIDDAATDQDLRVPPSNHFEKLRGNLAGLHSIRVNQQWRLIFRW
DGTRGEADGIYLDDHSYR

Nucleotide

• Length: 297 bp

>T95045 NZ_CP026525:c1155673-1155377 [Sinorhizobium meliloti]
GTGATCGTAGGCTTTCGGGACGACTGGCTTCGGACCTTCTTTGTGGATGATGTCCGTTCCCGTAACATCCCGTACGATCT
TGAAGCTCGGTTGTTCCGCAAGCTCCAGATGATTGACGATGCCGCAACCGACCAAGACTTGCGCGTGCCGCCCAGCAATC
ACTTCGAGAAGCTGCGCGGCAATCTGGCGGGGCTGCATTCGATCCGCGTCAACCAGCAATGGCGGCTGATCTTCCGCTGG
GATGGGACGCGCGGAGAGGCCGACGGGATTTATCTGGACGATCACAGCTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11588.44 Da
• Isoelectric Point: 11.0603

>AT95045 WP_010969010.1 NZ_CP026525:c1155363-1155055 [Sinorhizobium meliloti]
MLTTKRKPATVGEILTEEFMRPMGLTQGALAEAMGVQRKHVNELCGNRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
LVMNTPKERERIERARPLENAA

Nucleotide

• Length: 309 bp

>AT95045 NZ_CP026525:c1155363-1155055 [Sinorhizobium meliloti]
ATGCTGACCACCAAGCGCAAGCCGGCGACTGTCGGCGAGATACTGACTGAAGAGTTCATGCGGCCGATGGGATTGACGCA
GGGCGCGCTCGCCGAGGCGATGGGCGTCCAGCGCAAGCACGTCAACGAATTATGCGGCAACCGCAGGAACGTGACGGCGG
CGACGGCGCTGATCCTCGCCCGCGTGTTCGGCAACAGCCCGGACTTCTGGCTCAATGTGCAGAGGCGCAACGATCTTTGG
CTGGTGATGAATACGCCAAAGGAACGTGAGCGGATCGAGCGCGCGCGTCCGTTGGAGAATGCAGCCTGA

Structures

Toxin

• AlphaFold DB: Q92R61