Detailed information of TA system TA095007

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 159930..160355
• Replicon: plasmid pHS13-1-IncF
• Accession: NZ_CP026494
• Organism: Escherichia coli strain HS13-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: A0A0D8W959
• Locus tag: C3K24_RS28840
• Protein ID: WP_001332444.1
• Coordinates: 159930..160091 (-)
• Length: 54 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	160144..160355 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3K24_RS28805	155280..155507	-	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-
C3K24_RS28810	155601..156287	-	687	WP_000332490.1	PAS domain-containing protein	-
C3K24_RS28815	156477..156860	-	384	WP_000124981.1	relaxosome protein TraM	-
C3K24_RS28825	157194..157784	+	591	WP_000252676.1	transglycosylase SLT domain-containing protein	-
C3K24_RS28830	158080..158901	-	822	WP_001234485.1	DUF945 domain-containing protein	-
C3K24_RS28835	159080..159692	+	613	Protein_162	hypothetical protein	-
C3K24_RS28840	159930..160091	-	162	WP_001332444.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	160144..160355	-	212	NuclAT_0	-	Antitoxin
-	160144..160355	-	212	NuclAT_0	-	Antitoxin
-	160144..160355	-	212	NuclAT_0	-	Antitoxin
-	160144..160355	-	212	NuclAT_0	-	Antitoxin
C3K24_RS28850	160366..161085	-	720	WP_001276275.1	plasmid SOS inhibition protein A	-
C3K24_RS28855	161082..161516	-	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
C3K24_RS28860	161571..163529	-	1959	Protein_166	ParB/RepB/Spo0J family partition protein	-
C3K24_RS28865	163588..163821	-	234	WP_001209608.1	DUF905 domain-containing protein	-
C3K24_RS28870	163884..164381	-	498	WP_050427786.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / tet(A)	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..201203	201203
	-	flank	IS/Tn	-	-	165227..165730	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-48)

Sequences

Toxin

Protein

• Length: 54 a.a.
• Molecular weight: 5976.19 Da
• Isoelectric Point: 8.5101

>T95007 WP_001332444.1 NZ_CP026494:c160091-159930 [Escherichia coli]
MKLPGNALIWCVLIVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 162 bp

>T95007 NZ_CP026494:c160091-159930 [Escherichia coli]
ATGAAACTACCGGGAAACGCCCTTATCTGGTGCGTATTAATCGTGTGCTGCACGTTGTTAATATTCACACTCCTGACCCG
GAATCGTCTGTGCGAAGTCCGGCTGAAGGACGGATACAGGGAGGTTACGGCAAGTTTGGCTTACGAATCCAGCGGTAAGT
AG

Antitoxin

Nucleotide

• Length: 212 bp

>AT95007 NZ_CP026494:c160355-160144 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Toxin

• AlphaFold DB: A0A0D8W959

Antitoxin