Detailed information of TA system TA095004

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 122132..122386
• Replicon: plasmid pHS13-1-IncF
• Accession: NZ_CP026494
• Organism: Escherichia coli strain HS13-1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: C3K24_RS28610
• Protein ID: WP_001351576.1
• Coordinates: 122132..122338 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	122325..122386 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3K24_RS28575	117216..117776	-	561	WP_001161490.1	recombinase family protein	-
C3K24_RS28580	118086..119663	+	1578	WP_001323889.1	PAS domain-containing methyl-accepting chemotaxis protein	-
C3K24_RS28585	119960..120817	-	858	WP_019842754.1	incFII family plasmid replication initiator RepA	-
C3K24_RS28590	120810..121292	-	483	WP_001273588.1	hypothetical protein	-
C3K24_RS28595	121285..121359	-	75	WP_001442103.1	RepA leader peptide Tap	-
C3K24_RS28605	121591..121848	-	258	WP_000083845.1	replication regulatory protein RepA	-
C3K24_RS28610	122132..122338	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	122325..122386	+	62	NuclAT_1	-	Antitoxin
-	122325..122386	+	62	NuclAT_1	-	Antitoxin
-	122325..122386	+	62	NuclAT_1	-	Antitoxin
-	122325..122386	+	62	NuclAT_1	-	Antitoxin
C3K24_RS29660	122642..122716	-	75	Protein_120	endonuclease	-
C3K24_RS28620	122962..123174	-	213	WP_001299730.1	ANR family transcriptional regulator	-
C3K24_RS28625	123310..123870	-	561	WP_000139315.1	fertility inhibition protein FinO	-
C3K24_RS28630	123973..124833	-	861	WP_000704513.1	alpha/beta hydrolase	-
C3K24_RS28635	124892..125638	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD / tet(A)	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..201203	201203
	-	inside	IScluster/Tn	tet(A)	-	103173..117776	14603

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T95004 WP_001351576.1 NZ_CP026494:c122338-122132 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T95004 NZ_CP026494:c122338-122132 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT95004 NZ_CP026494:122325-122386 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin