Detailed information of TA system TA094992

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3978175..3978869
• Replicon: chromosome
• Accession: NZ_CP026491
• Organism: Escherichia coli strain HS13-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: C3K24_RS20480
• Protein ID: WP_001263500.1
• Coordinates: 3978471..3978869 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: C3K24_RS20475
• Protein ID: WP_000554758.1
• Coordinates: 3978175..3978468 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3K24_RS20455	3973711..3974208	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
C3K24_RS29260	3974291..3974449	-	159	WP_014639450.1	hypothetical protein	-
C3K24_RS20460	3974528..3976240	-	1713	Protein_3761	flagellar biosynthesis protein FlhA	-
C3K24_RS20465	3976212..3976997	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
C3K24_RS20470	3977068..3978123	+	1056	WP_001226168.1	DNA polymerase IV	-
C3K24_RS20475	3978175..3978468	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C3K24_RS20480	3978471..3978869	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C3K24_RS20485	3978879..3979331	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
C3K24_RS20490	3979521..3980660	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
C3K24_RS20495	3980657..3981271	+	615	WP_000602123.1	peptide chain release factor H	-
C3K24_RS20500	3981328..3982785	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
C3K24_RS20505	3983046..3983504	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3958650..3979331	20681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T94992 WP_001263500.1 NZ_CP026491:3978471-3978869 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T94992 NZ_CP026491:3978471-3978869 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT94992 WP_000554758.1 NZ_CP026491:3978175-3978468 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT94992 NZ_CP026491:3978175-3978468 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3