Detailed information of TA system TA094985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3007933..3008535
• Replicon: chromosome
• Accession: NZ_CP026491
• Organism: Escherichia coli strain HS13-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: C3K24_RS15585
• Protein ID: WP_000897302.1
• Coordinates: 3007933..3008244 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C3K24_RS15590
• Protein ID: WP_000356397.1
• Coordinates: 3008245..3008535 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3K24_RS15560	3003847..3004446	+	600	WP_001314324.1	glucose-1-phosphatase	-
C3K24_RS15565	3004440..3005312	+	873	WP_000920754.1	virulence factor BrkB family protein	-
C3K24_RS15570	3005309..3005746	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
C3K24_RS15575	3005791..3006732	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
C3K24_RS15580	3006796..3007704	-	909	WP_061091392.1	alpha/beta hydrolase	-
C3K24_RS15585	3007933..3008244	+	312	WP_000897302.1	hypothetical protein	Toxin
C3K24_RS15590	3008245..3008535	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
C3K24_RS15600	3008894..3009172	+	279	WP_001296612.1	hypothetical protein	-
C3K24_RS15610	3009568..3009786	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
C3K24_RS15615	3010002..3010931	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
C3K24_RS15620	3010928..3011563	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
C3K24_RS15625	3011560..3012462	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T94985 WP_000897302.1 NZ_CP026491:3007933-3008244 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T94985 NZ_CP026491:3007933-3008244 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT94985 WP_000356397.1 NZ_CP026491:3008245-3008535 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT94985 NZ_CP026491:3008245-3008535 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416