Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 818853..819355 Replicon chromosome
Accession NZ_CP026491
Organism Escherichia coli strain HS13-1

Toxin (Protein)


Gene name yoeB Uniprot ID A0A0H2Z0A6
Locus tag C3K24_RS04505 Protein ID WP_000767826.1
Coordinates 818853..819107 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C3K24_RS04510 Protein ID WP_001259255.1
Coordinates 819104..819355 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3K24_RS04475 813868..814095 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
C3K24_RS04480 814109..815167 - 1059 WP_000492334.1 transport protein YeeE -
C3K24_RS04485 815346..816704 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C3K24_RS04490 816694..816756 - 63 WP_010723108.1 membrane protein YoeI -
C3K24_RS04495 816971..817900 - 930 WP_000803363.1 LysR family transcriptional regulator -
C3K24_RS04500 817946..818770 - 825 WP_000754737.1 SDR family oxidoreductase -
C3K24_RS04505 818853..819107 - 255 WP_000767826.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C3K24_RS04510 819104..819355 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C3K24_RS04515 819638..819679 + 42 WP_010989201.1 his operon leader peptide -
C3K24_RS04520 819825..820724 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C3K24_RS04525 820730..822034 + 1305 WP_001350692.1 histidinol dehydrogenase -
C3K24_RS04530 822031..823101 + 1071 WP_000108997.1 histidinol-phosphate transaminase -
C3K24_RS04535 823101..824168 + 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10195.64 Da        Isoelectric Point: 8.0353

>T94981 WP_000767826.1 NZ_CP026491:c819107-818853 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRIIEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T94981 NZ_CP026491:c819107-818853 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTATAGAGGAACACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT94981 WP_001259255.1 NZ_CP026491:c819355-819104 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT94981 NZ_CP026491:c819355-819104 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCACCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2Z0A6

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References