Detailed information of TA system TA094967

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 103808..104047
• Replicon: plasmid pKBN10P04869A
• Accession: NZ_CP026474
• Organism: Escherichia coli strain KBN10P04869

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: YKEC1_RS25550
• Protein ID: WP_023144756.1
• Coordinates: 103913..104047 (+)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	103808..103868 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YKEC1_RS25520	98925..101318	+	2394	Protein_113	AAA family ATPase	-
YKEC1_RS25525	101338..102084	+	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
YKEC1_RS25530	102139..102699	+	561	WP_000139341.1	fertility inhibition protein FinO	-
YKEC1_RS25535	102830..103042	+	213	WP_013023861.1	hypothetical protein	-
YKEC1_RS26905	103555..103841	+	287	Protein_117	DUF2726 domain-containing protein	-
-	103808..103868	-	61	NuclAT_0	-	Antitoxin
-	103808..103868	-	61	NuclAT_0	-	Antitoxin
-	103808..103868	-	61	NuclAT_0	-	Antitoxin
-	103808..103868	-	61	NuclAT_0	-	Antitoxin
YKEC1_RS25550	103913..104047	+	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
YKEC1_RS25555	104344..104598	+	255	WP_000083850.1	replication regulatory protein RepA	-
YKEC1_RS25560	104758..105504	-	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
YKEC1_RS25565	105519..107060	-	1542	WP_002431311.1	IS21-like element ISEc12 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / mph(A) / aac(3)-IId / aadA5 / qacE / sul1 / blaNDM-5 / dfrA12 / aadA2 / tet(B)	-	1..107229	107229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T94967 WP_023144756.1 NZ_CP026474:103913-104047 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T94967 NZ_CP026474:103913-104047 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT94967 NZ_CP026474:c103868-103808 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin