Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1836038..1836540 Replicon chromosome
Accession NZ_CP026473
Organism Escherichia coli strain KBN10P04869

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag YKEC1_RS09325 Protein ID WP_000767829.1
Coordinates 1836286..1836540 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag YKEC1_RS09320 Protein ID WP_001259255.1
Coordinates 1836038..1836289 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
YKEC1_RS09290 1831215..1832282 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
YKEC1_RS09295 1832282..1833352 - 1071 WP_053908449.1 histidinol-phosphate transaminase -
YKEC1_RS09300 1833349..1834653 - 1305 WP_053908451.1 histidinol dehydrogenase -
YKEC1_RS09305 1834659..1835558 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
YKEC1_RS09315 1835704..1835754 - 51 WP_001364200.1 his operon leader peptide -
YKEC1_RS09320 1836038..1836289 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
YKEC1_RS09325 1836286..1836540 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
YKEC1_RS09330 1836623..1837447 + 825 WP_053908453.1 SDR family oxidoreductase -
YKEC1_RS09335 1837493..1838422 + 930 WP_053908455.1 LysR family transcriptional regulator -
YKEC1_RS09340 1838637..1838699 + 63 WP_010723108.1 membrane protein YoeI -
YKEC1_RS09345 1838689..1840047 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
YKEC1_RS09350 1840226..1841284 + 1059 WP_053908457.1 transport protein YeeE -
YKEC1_RS09355 1841298..1841525 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T94950 WP_000767829.1 NZ_CP026473:1836286-1836540 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T94950 NZ_CP026473:1836286-1836540 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT94950 WP_001259255.1 NZ_CP026473:1836038-1836289 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT94950 NZ_CP026473:1836038-1836289 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References