Detailed information of TA system TA094850

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 81113..81764
• Replicon: plasmid pACI-df08
• Accession: NZ_CP026426
• Organism: Acinetobacter sp. ACNIH1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8WRB6
• Locus tag: C3F22_RS17170
• Protein ID: WP_004780010.1
• Coordinates: 81408..81764 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C3F22_RS17165
• Protein ID: WP_004780009.1
• Coordinates: 81113..81415 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F22_RS17140	76151..77776	-	1626	WP_005104480.1	IS66 family transposase	-
C3F22_RS17145	77851..78186	-	336	WP_004642106.1	IS66 family insertion sequence element accessory protein TnpB	-
C3F22_RS17150	78183..78566	-	384	WP_005100737.1	IS66 family insertion sequence hypothetical protein	-
C3F22_RS17155	78653..79078	+	426	Protein_79	LysE family translocator	-
C3F22_RS17160	79388..80962	-	1575	WP_004780008.1	sensor domain-containing diguanylate cyclase	-
C3F22_RS17165	81113..81415	-	303	WP_004780009.1	XRE family transcriptional regulator	Antitoxin
C3F22_RS17170	81408..81764	-	357	WP_004780010.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3F22_RS17175	81879..82193	+	315	WP_004812036.1	hypothetical protein	-
C3F22_RS17180	82229..82933	-	705	WP_001067784.1	IS6-like element IS1006 family transposase	-
C3F22_RS17185	83091..83990	-	900	WP_004733183.1	cation diffusion facilitator family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..257654	257654
	-	inside	IScluster/Tn	-	-	73261..82933	9672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (13-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13575.59 Da
• Isoelectric Point: 7.1654

>T94850 WP_004780010.1 NZ_CP026426:c81764-81408 [Acinetobacter sp. ACNIH1]
MWTVITTDLFNKWLEQQDESTQEKVLAALVVLQQQGPSLRRPLVDIVYDSKFANMKELRVQHRGKSLRAFFAFDPLRQAI
VLCIGDKSGKKRFYTEMLAIADEQYELHLSTLGDQSNG

Nucleotide

• Length: 357 bp

>T94850 NZ_CP026426:c81764-81408 [Acinetobacter sp. ACNIH1]
ATGTGGACAGTCATTACAACGGATCTGTTTAATAAATGGCTTGAACAGCAAGATGAATCAACACAAGAGAAAGTCTTGGC
TGCACTGGTTGTACTTCAACAGCAAGGACCAAGTTTAAGGCGTCCCTTAGTCGATATCGTCTACGACTCTAAATTTGCCA
ACATGAAGGAACTTCGTGTCCAACATCGAGGTAAATCACTCAGGGCATTTTTTGCCTTTGATCCATTACGACAGGCTATT
GTGCTTTGTATTGGTGATAAGAGCGGTAAAAAGCGTTTTTATACAGAGATGCTAGCAATTGCAGATGAGCAATACGAACT
TCATCTCTCAACTTTAGGAGATCAATCAAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11253.01 Da
• Isoelectric Point: 5.1991

>AT94850 WP_004780009.1 NZ_CP026426:c81415-81113 [Acinetobacter sp. ACNIH1]
MAKTLQELLASRSPESQARIQQMADELLLENQLYLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT94850 NZ_CP026426:c81415-81113 [Acinetobacter sp. ACNIH1]
ATGGCTAAGACCCTACAAGAATTATTAGCTAGTCGCTCTCCAGAGAGCCAAGCTCGTATTCAACAAATGGCGGATGAGTT
ATTGCTAGAAAATCAGCTTTATCTGATTCGTGAAGAATTAGAGATTTCCCAAAAAGAATTGGCTGAAACCCTTGGTATCA
AACAGCCATCTCTTTCCGCAATCGAAAATCGTGGGAATGATCTAAAAATTTCAACTATGAAAAAATATGTTGAAGCGATG
GGTGGAAAATTACGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTATAA

Structures

Toxin

• AlphaFold DB: N8WRB6