Detailed information of TA system TA094846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 5846..6431
• Replicon: plasmid pKPC-8dee
• Accession: NZ_CP026418
• Organism: Acinetobacter sp. ACNIH2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: C3F34_RS19760
• Protein ID: WP_000897307.1
• Coordinates: 5846..6166 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C3F34_RS19765
• Protein ID: WP_000369781.1
• Coordinates: 6159..6431 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F34_RS19725	1268..2755	-	1488	WP_104442463.1	SulP family inorganic anion transporter	-
C3F34_RS19730	3267..3554	+	288	WP_000438825.1	BrnT family toxin	-
C3F34_RS19735	3541..3855	+	315	WP_004728120.1	BrnA antitoxin family protein	-
C3F34_RS19740	3944..4474	-	531	WP_000172759.1	hypothetical protein	-
C3F34_RS19745	4623..4925	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
C3F34_RS19750	4926..5207	+	282	WP_000985609.1	putative addiction module antidote protein	-
C3F34_RS19755	5287..5706	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
C3F34_RS19760	5846..6166	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3F34_RS19765	6159..6431	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
C3F34_RS19770	6924..8399	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C3F34_RS19775	8551..9213	-	663	WP_104440784.1	IS1595 family transposase	-
C3F34_RS19780	9254..10078	+	825	WP_104442464.1	Mph(E) family macrolide 2'-phosphotransferase	-
C3F34_RS19785	10268..10879	-	612	WP_015060246.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / tet(39) / blaKPC-2 / blaGES-7 / qacE / sul1	-	1..43928	43928
	-	flank	IS/Tn	msr(E) / mph(E) / tet(39)	-	6924..12292	5368

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T94846 WP_000897307.1 NZ_CP026418:5846-6166 [Acinetobacter sp. ACNIH2]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T94846 NZ_CP026418:5846-6166 [Acinetobacter sp. ACNIH2]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT94846 WP_000369781.1 NZ_CP026418:6159-6431 [Acinetobacter sp. ACNIH2]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT94846 NZ_CP026418:6159-6431 [Acinetobacter sp. ACNIH2]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3