Detailed information of TA system TA094843

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 7211..7796
• Replicon: plasmid pACI-c6b4
• Accession: NZ_CP026417
• Organism: Acinetobacter sp. ACNIH2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: C3F34_RS19680
• Protein ID: WP_000897307.1
• Coordinates: 7211..7531 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C3F34_RS19685
• Protein ID: WP_000369781.1
• Coordinates: 7524..7796 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F34_RS20440	3180..3350	-	171	WP_171263913.1	hypothetical protein	-
C3F34_RS19660	3347..3733	-	387	WP_032001883.1	hypothetical protein	-
C3F34_RS19665	4910..5833	+	924	WP_032066855.1	replication initiation protein RepM	-
C3F34_RS19670	5897..6463	+	567	WP_032024465.1	plasmid replication DNA-binding protein	-
C3F34_RS19675	6652..7071	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
C3F34_RS19680	7211..7531	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3F34_RS19685	7524..7796	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
C3F34_RS19690	8289..9764	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C3F34_RS19695	9820..10704	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
C3F34_RS19700	10894..11505	-	612	WP_015060246.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / tet(39)	-	1..12933	12933

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T94843 WP_000897307.1 NZ_CP026417:7211-7531 [Acinetobacter sp. ACNIH2]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T94843 NZ_CP026417:7211-7531 [Acinetobacter sp. ACNIH2]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT94843 WP_000369781.1 NZ_CP026417:7524-7796 [Acinetobacter sp. ACNIH2]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT94843 NZ_CP026417:7524-7796 [Acinetobacter sp. ACNIH2]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3