Detailed information of TA system TA094842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 56105..56756
• Replicon: plasmid pACI-55cf
• Accession: NZ_CP026415
• Organism: Acinetobacter sp. ACNIH2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2L0I0R9
• Locus tag: C3F34_RS18625
• Protein ID: WP_019383563.1
• Coordinates: 56400..56756 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C3F34_RS18620
• Protein ID: WP_001140620.1
• Coordinates: 56105..56407 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F34_RS18590	51368..51694	+	327	WP_016165445.1	DUF3240 family protein	-
C3F34_RS18595	51691..52998	+	1308	WP_104442406.1	TolC family protein	-
C3F34_RS18600	53238..53900	+	663	WP_092692964.1	response regulator	-
C3F34_RS18605	53900..55255	+	1356	WP_016165442.1	two-component sensor histidine kinase	-
C3F34_RS18610	55533..55719	-	187	Protein_49	IS256 family transposase	-
C3F34_RS18615	55902..56063	+	162	Protein_50	IS6 family transposase	-
C3F34_RS18620	56105..56407	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
C3F34_RS18625	56400..56756	-	357	WP_019383563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3F34_RS18630	57474..57932	+	459	WP_019383562.1	TIR domain-containing protein	-
C3F34_RS18635	57932..58432	+	501	Protein_54	hypothetical protein	-
C3F34_RS18645	59619..59954	+	336	Protein_56	hypothetical protein	-
C3F34_RS18650	60026..60160	-	135	Protein_57	IS481 family transposase	-
C3F34_RS18655	60482..60937	-	456	WP_019383558.1	protein-export chaperone SecB	-
C3F34_RS18660	60934..61500	-	567	WP_019383557.1	type II toxin-antitoxin system MqsA family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..118501	118501
	-	inside	IScluster/Tn	-	-	55902..59544	3642

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13591.59 Da
• Isoelectric Point: 6.7180

>T94842 WP_019383563.1 NZ_CP026415:c56756-56400 [Acinetobacter sp. ACNIH2]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGHPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T94842 NZ_CP026415:c56756-56400 [Acinetobacter sp. ACNIH2]
ATGTGGACAGTCATTACGACAGATCTATTTAATCAGTGGCTTGAACAGCAAGATGAACCTACACAAGAGAAGGTCCTAGC
TGCCCTGGTTGTTCTACAGCAGCAGGGACCGAGTTTAGGCCATCCTTTAGTAGATACTGTGTATGAGTCTAAATTTACCA
ATATGAAAGAACTGCGTGTTCAACATCGCGGTAGACCCTTAAGAGCTTTCTTCGCATTTGATCCCTTACGACAGGCTATT
GTTCTATGTATTGCTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGACATGCTGGATATTGCAGATGAACAATACCAACT
TCATCTCACTACCCTAGGAGATAAATCTAATGGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT94842 WP_001140620.1 NZ_CP026415:c56407-56105 [Acinetobacter sp. ACNIH2]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT94842 NZ_CP026415:c56407-56105 [Acinetobacter sp. ACNIH2]
ATGGCTAAGACTCTGCAAGAATTGTTAGCTAGCCGCTCTCCAGAGAGCCAAGCCCGTATTCAAAAAATGGCTGATGAATT
ACTGCTAGAAAATCAGCTTCATCTTATTCGTGAAGAACTCGAAATTTCTCAAAAAGAGCTTGCTGAAACTTTGGGAATAA
AACAGCCATCGCTTTCAGCAATAGAAAATCGTGGCAATGATCTTAAGATTTCAACCATGAAAAAGTATGTTGAGGCAATG
GGTGGGAAGCTCCGTATTGATGTAGAGCTTCCAACAGGAAAACATATAGGATTCAACGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2L0I0R9