Detailed information of TA system TA094841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 7270..7855
• Replicon: plasmid pACI-235c
• Accession: NZ_CP026414
• Organism: Acinetobacter sp. ACNIH2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: C3F34_RS18300
• Protein ID: WP_000897307.1
• Coordinates: 7535..7855 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C3F34_RS18295
• Protein ID: WP_000369781.1
• Coordinates: 7270..7542 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F34_RS18280	3561..4172	+	612	WP_015060246.1	recombinase family protein	-
C3F34_RS18285	4362..5246	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
C3F34_RS18290	5302..6777	-	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
C3F34_RS18295	7270..7542	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
C3F34_RS18300	7535..7855	-	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3F34_RS18305	7995..8414	+	420	WP_001180106.1	SMI1/KNR4 family protein	-
C3F34_RS18310	8682..8957	+	276	WP_104442390.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
C3F34_RS18315	8935..9264	+	330	WP_104442391.1	type II toxin-antitoxin system YafQ family toxin	-
C3F34_RS18320	9289..9804	+	516	WP_104442392.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(39) / mph(E) / msr(E)	-	1..17598	17598

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T94841 WP_000897307.1 NZ_CP026414:c7855-7535 [Acinetobacter sp. ACNIH2]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T94841 NZ_CP026414:c7855-7535 [Acinetobacter sp. ACNIH2]
ATGTTATTTATTGAAACCAGCATCTTTACCAAGCAAATTAAAGACCTTGTATCTGATGAGGAATATCGTCAACTTCAGCA
AGATCTTTTGGTACAGCCTGATAGAGGTGACCTAATCAAGAATGGCGGTGGTATTCGCAAAGTACGTTGTGCTCAAGGTA
ACAAAGGGAAAAGTGGTGGGATACGGGTGATTTATTATTGGGTCACCGAGGATGATCAGATCTTTTTCTTAGTGGCTTAT
CCAAAATCAGTAAAAGATAATTTAACAGATAAGGAAACCGCCATTCTGCACCAACTCGTGAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT94841 WP_000369781.1 NZ_CP026414:c7542-7270 [Acinetobacter sp. ACNIH2]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT94841 NZ_CP026414:c7542-7270 [Acinetobacter sp. ACNIH2]
ATGGATAACAACTTATTTGATGACTTGGTTGCTTCAATCAAAGAAGCTGGAGCTATCAAACGTAAAGAAGTTAAAGCAAG
CCGAGTTACAGAACTCGAATTGCCTGATATTAAAGAAGTACGTGAAAAAACTGGCTTGAGCCAAAATGAATTTGCTGCAC
GTCTACATATTAGCCCCCGTACCCTGCAAAATTGGGAACAGGGACGACGCTATCCAACTGGACCAGCAGCTACATTGATT
CGAATTTTAGATGCTCACCCAAGCCTTATTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3