Detailed information of TA system TA094840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2831982..2832572
• Replicon: chromosome
• Accession: NZ_CP026412
• Organism: Acinetobacter sp. ACNIH2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C3F34_RS13370
• Protein ID: WP_044738568.1
• Coordinates: 2831982..2832263 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: C3F34_RS13375
• Protein ID: WP_104441814.1
• Coordinates: 2832288..2832572 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F34_RS13345	2827536..2828483	+	948	WP_104441810.1	hypothetical protein	-
C3F34_RS13350	2828900..2829975	+	1076	WP_104440995.1	IS3 family transposase	-
C3F34_RS13355	2830104..2830529	-	426	WP_104441811.1	hypothetical protein	-
C3F34_RS13360	2830613..2830909	-	297	WP_104441812.1	hypothetical protein	-
C3F34_RS13365	2831212..2831721	-	510	WP_104441813.1	hypothetical protein	-
C3F34_RS13370	2831982..2832263	+	282	WP_044738568.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C3F34_RS13375	2832288..2832572	+	285	WP_104441814.1	HigA family addiction module antidote protein	Antitoxin
C3F34_RS13380	2832621..2833106	-	486	WP_104441815.1	hypothetical protein	-
C3F34_RS13385	2833075..2833722	-	648	WP_158654360.1	hypothetical protein	-
C3F34_RS13390	2833863..2834069	-	207	WP_104441817.1	excalibur calcium-binding domain-containing protein	-
C3F34_RS13395	2834154..2835244	+	1091	WP_104442269.1	IS4 family transposase	-
C3F34_RS13400	2835460..2836113	+	654	WP_104441818.1	hypothetical protein	-
C3F34_RS13405	2836211..2836468	+	258	WP_104441819.1	hypothetical protein	-
C3F34_RS13410	2836546..2837316	-	771	WP_158654361.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2785701..2848833	63132
	-	inside	IScluster/Tn	-	-	2828900..2835244	6344

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (11-66)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10568.07 Da
• Isoelectric Point: 9.9524

>T94840 WP_044738568.1 NZ_CP026412:2831982-2832263 [Acinetobacter sp. ACNIH2]
MIKSFKHKGLQAFFQTGTTAGIQAAHSAKLRLILAALHAASTVNDLRTPPNWRLHKLSGNLQDQWSLTVNGNWRVIFKFE
DGDVYIVDYLDYH

Nucleotide

• Length: 282 bp

>T94840 NZ_CP026412:2831982-2832263 [Acinetobacter sp. ACNIH2]
TTGATTAAGAGTTTTAAACATAAAGGTCTTCAGGCTTTTTTCCAAACTGGTACAACAGCAGGTATTCAAGCAGCGCATTC
TGCCAAATTACGTTTAATCCTTGCAGCATTACATGCTGCTTCCACAGTGAATGATTTAAGAACTCCACCAAATTGGCGTT
TGCACAAGTTAAGTGGAAATCTACAAGATCAATGGTCACTAACTGTAAACGGTAATTGGCGGGTAATTTTTAAATTTGAA
GATGGTGATGTCTACATTGTGGATTATCTGGACTATCACTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10635.19 Da
• Isoelectric Point: 6.7228

>AT94840 WP_104441814.1 NZ_CP026412:2832288-2832572 [Acinetobacter sp. ACNIH2]
MNMMFNAPHPGEMLREYIGETPVTEAAQALGVTRANLSRILNGHTGISADMAIRLSEALETSPEFWLNLQMQHDLWNASQ
RQRPVIKKLVQNFT

Nucleotide

• Length: 285 bp

>AT94840 NZ_CP026412:2832288-2832572 [Acinetobacter sp. ACNIH2]
ATGAATATGATGTTCAATGCACCACATCCTGGTGAAATGTTACGGGAATACATTGGTGAAACTCCTGTGACGGAGGCTGC
TCAAGCACTAGGGGTTACTCGTGCAAATCTATCTCGTATTTTGAATGGTCATACTGGTATTTCAGCAGATATGGCAATTC
GTTTAAGCGAGGCTTTGGAAACATCACCAGAGTTTTGGTTAAATTTGCAGATGCAGCATGACTTATGGAATGCCAGTCAA
CGTCAGAGACCAGTCATAAAGAAATTGGTTCAGAATTTTACATAA