Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 27306..28033 Replicon plasmid pECO-6357
Accession NZ_CP026404
Organism Escherichia coli strain ECONIH4

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag C3F40_RS29350 Protein ID WP_000558568.1
Coordinates 27306..27617 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag C3F40_RS29355 Protein ID WP_000990392.1
Coordinates 27614..28033 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F40_RS29335 23069..23329 + 261 Protein_24 recombinase family protein -
C3F40_RS29340 23386..24090 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
C3F40_RS29345 24155..27076 + 2922 Protein_26 Tn3 family transposase -
C3F40_RS29350 27306..27617 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
C3F40_RS29355 27614..28033 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
C3F40_RS29360 28070..28279 - 210 WP_020806042.1 hypothetical protein -
C3F40_RS29365 29019..29723 - 705 WP_061872725.1 IS6-like element IS26 family transposase -
C3F40_RS29375 30114..30811 - 698 Protein_31 IS1 family transposase -
C3F40_RS29380 30868..31086 - 219 Protein_32 hypothetical protein -
C3F40_RS29385 31619..31954 + 336 WP_001039462.1 hypothetical protein -
C3F40_RS29390 31963..32154 + 192 WP_000861578.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..127217 127217
- inside IScluster/Tn - - 20338..34518 14180


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T94832 WP_000558568.1 NZ_CP026404:27306-27617 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T94832 NZ_CP026404:27306-27617 [Escherichia coli]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT94832 WP_000990392.1 NZ_CP026404:27614-28033 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT94832 NZ_CP026404:27614-28033 [Escherichia coli]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References