Detailed information of TA system TA094827

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 37820..38090
• Replicon: plasmid pECO-816c
• Accession: NZ_CP026403
• Organism: Escherichia coli strain ECONIH4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C3F40_RS28800
• Protein ID: WP_001312861.1
• Coordinates: 37932..38090 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	37820..37883 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C3F40_RS31625	33356..33562	+	207	WP_000275853.1	hypothetical protein	-
C3F40_RS28775	33588..34127	+	540	WP_000290838.1	single-stranded DNA-binding protein	-
C3F40_RS28780	34190..34423	+	234	WP_000005990.1	DUF905 family protein	-
C3F40_RS28785	34489..36447	+	1959	WP_097734687.1	ParB/RepB/Spo0J family partition protein	-
C3F40_RS28790	36502..36936	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C3F40_RS28795	36933..37652	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
C3F40_RS31385	37664..37852	-	189	WP_001299721.1	hypothetical protein	-
-	37664..37888	+	225	NuclAT_0	-	-
-	37664..37888	+	225	NuclAT_0	-	-
-	37664..37888	+	225	NuclAT_0	-	-
-	37664..37888	+	225	NuclAT_0	-	-
-	37820..37883	-	64	-	-	Antitoxin
C3F40_RS28800	37932..38090	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
C3F40_RS28820	39012..39299	+	288	WP_000107548.1	hypothetical protein	-
C3F40_RS28825	39419..40240	+	822	WP_139942663.1	DUF945 domain-containing protein	-
C3F40_RS28830	40537..41139	-	603	WP_077815103.1	transglycosylase SLT domain-containing protein	-
C3F40_RS28835	41460..41843	+	384	WP_001151524.1	relaxosome protein TraM	-
C3F40_RS28840	42030..42719	+	690	WP_097734686.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..103161	103161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T94827 WP_001312861.1 NZ_CP026403:37932-38090 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T94827 NZ_CP026403:37932-38090 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT94827 NZ_CP026403:c37883-37820 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin