Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 5254263..5254765 Replicon chromosome
Accession NZ_CP026399
Organism Escherichia coli strain ECONIH4

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C3F40_RS27740 Protein ID WP_000767829.1
Coordinates 5254511..5254765 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C3F40_RS27735 Protein ID WP_001259255.1
Coordinates 5254263..5254514 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F40_RS27705 5249441..5250508 - 1068 WP_000080097.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C3F40_RS27710 5250508..5251578 - 1071 WP_000108970.1 histidinol-phosphate transaminase -
C3F40_RS27715 5251575..5252879 - 1305 WP_032218856.1 histidinol dehydrogenase -
C3F40_RS27720 5252885..5253784 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
C3F40_RS27730 5253930..5253980 - 51 WP_001364200.1 his operon leader peptide -
C3F40_RS27735 5254263..5254514 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C3F40_RS27740 5254511..5254765 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C3F40_RS27745 5254848..5255672 + 825 WP_000754758.1 SDR family oxidoreductase -
C3F40_RS27750 5255718..5256647 + 930 WP_000803351.1 LysR family transcriptional regulator -
C3F40_RS27755 5256862..5256924 + 63 WP_010723108.1 membrane protein YoeI -
C3F40_RS27760 5256914..5258272 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C3F40_RS27765 5258451..5259509 + 1059 WP_000492312.1 transport protein YeeE -
C3F40_RS27770 5259523..5259750 + 228 WP_000985278.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T94823 WP_000767829.1 NZ_CP026399:5254511-5254765 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T94823 NZ_CP026399:5254511-5254765 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT94823 WP_001259255.1 NZ_CP026399:5254263-5254514 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT94823 NZ_CP026399:5254263-5254514 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References