Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 1464239..1464836 Replicon chromosome
Accession NZ_CP026392
Organism Klebsiella pneumoniae strain KPNIH48

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag C3F39_RS07635 Protein ID WP_004142563.1
Coordinates 1464519..1464836 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag C3F39_RS07630 Protein ID WP_004142561.1
Coordinates 1464239..1464526 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3F39_RS07600 1460319..1460567 + 249 WP_002893037.1 DUF1158 domain-containing protein -
C3F39_RS07605 1460585..1460926 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
C3F39_RS07610 1460957..1462072 - 1116 WP_012737592.1 MBL fold metallo-hydrolase -
C3F39_RS07615 1462252..1462833 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
C3F39_RS07620 1462833..1463201 + 369 WP_040236580.1 MmcQ/YjbR family DNA-binding protein -
C3F39_RS07625 1463321..1463974 + 654 WP_004176973.1 oxygen-insensitive NAD(P)H nitroreductase -
C3F39_RS07630 1464239..1464526 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
C3F39_RS07635 1464519..1464836 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C3F39_RS07640 1465021..1466064 - 1044 WP_040236579.1 DUF2157 domain-containing protein -
C3F39_RS07650 1466734..1467600 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
C3F39_RS07655 1467709..1469136 + 1428 WP_040236578.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T94770 WP_004142563.1 NZ_CP026392:c1464836-1464519 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T94770 NZ_CP026392:c1464836-1464519 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT94770 WP_004142561.1 NZ_CP026392:c1464526-1464239 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT94770 NZ_CP026392:c1464526-1464239 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGGGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References