Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3277002..3277801 Replicon chromosome
Accession NZ_CP026353
Organism Escherichia coli strain 4A

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag C2R10_RS17030 Protein ID WP_000347273.1
Coordinates 3277337..3277801 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag C2R10_RS17025 Protein ID WP_001307405.1
Coordinates 3277002..3277337 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2R10_RS17010 3272787..3273557 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
C2R10_RS17015 3273573..3274907 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
C2R10_RS17020 3275282..3276853 + 1572 WP_001273753.1 galactarate dehydratase -
C2R10_RS17025 3277002..3277337 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
C2R10_RS17030 3277337..3277801 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
C2R10_RS17035 3277856..3278665 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
C2R10_RS17045 3278914..3280194 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
C2R10_RS17050 3280217..3280690 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
C2R10_RS17055 3280701..3281072 + 372 Protein_3118 PTS sugar transporter subunit IIC -
C2R10_RS17060 3281068..3281625 + 558 Protein_3119 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3267854..3288785 20931


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T94351 WP_000347273.1 NZ_CP026353:3277337-3277801 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T94351 NZ_CP026353:3277337-3277801 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT94351 WP_001307405.1 NZ_CP026353:3277002-3277337 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT94351 NZ_CP026353:3277002-3277337 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References