Detailed information of TA system TA094328

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 1413556..1413927
• Replicon: chromosome
• Accession: NZ_CP026353
• Organism: Escherichia coli strain 4A

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: C2R10_RS07445
• Protein ID: WP_001317028.1
• Coordinates: 1413733..1413927 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	1413556..1413734 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2R10_RS07420	1409511..1410884	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
C2R10_RS07425	1411013..1411948	-	936	WP_001157406.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
C2R10_RS07430	1412000..1413235	-	1236	WP_000040852.1	site-specific integrase	-
C2R10_RS07435	1413237..1413452	-	216	WP_000079604.1	excisionase XisR	-
C2R10_RS07440	1413531..1413740	-	210	WP_000276809.1	double-strand break reduction protein RcbA	-
-	1413556..1413734	+	179	NuclAT_0	-	Antitoxin
-	1413556..1413734	+	179	NuclAT_0	-	Antitoxin
-	1413556..1413734	+	179	NuclAT_0	-	Antitoxin
-	1413556..1413734	+	179	NuclAT_0	-	Antitoxin
C2R10_RS07445	1413733..1413927	-	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C2R10_RS07450	1413984..1414793	-	810	WP_000166319.1	recombination protein RecT	-
C2R10_RS07455	1414786..1417386	-	2601	WP_000105143.1	exodeoxyribonuclease VIII	-
C2R10_RS07460	1417488..1417763	-	276	WP_000632297.1	protein RacC	-
C2R10_RS07465	1417838..1418008	-	171	WP_001352098.1	conserved protein, Rac prophage	-
C2R10_RS07470	1418008..1418229	-	222	WP_000560225.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1380821..1433674	52853

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T94328 WP_001317028.1 NZ_CP026353:c1413927-1413733 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T94328 NZ_CP026353:c1413927-1413733 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT94328 NZ_CP026353:1413556-1413734 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin