Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/RHH-PIN
Location 1574112..1574767 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA7
Locus tag Rv1397c Protein ID NP_215913.1
Coordinates 1574112..1574513 (-) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WLZ1
Locus tag Rv1398c Protein ID NP_215914.1
Coordinates 1574510..1574767 (-) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv1394c 1569584..1570969 - 1386 YP_177807.1 cytochrome P450 Cyp132 -
Rv1395 1571047..1572081 + 1035 YP_177808.1 HTH-type transcriptional regulator -
Rv1396c 1572127..1573857 - 1731 YP_177809.1 PE-PGRS family protein PE_PGRS25 -
Rv1397c 1574112..1574513 - 402 NP_215913.1 ribonuclease VapC10 Toxin
Rv1398c 1574510..1574767 - 258 NP_215914.1 antitoxin VapB10 Antitoxin
Rv1399c 1574850..1575809 - 960 NP_215915.1 carboxylesterase NlhH -
Rv1400c 1575834..1576796 - 963 NP_215916.1 lipase -
Rv1401 1576930..1577532 + 603 NP_215917.1 membrane protein -
Rv1402 1577613..1579580 + 1968 NP_215918.1 primosomal protein N' -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-108)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-39)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14952.43 Da        Isoelectric Point: 11.4656

>T94 NP_215913.1 NC_000962:c1574513-1574112 [Mycobacterium tuberculosis H37Rv]
MILVDSDVLIAHLRGVVAARDWLVSARKDGPLAISVVSTAELIGGMRTAERREVWRLLASFRVQPATEVIARRAGDMMRR
YRRSHNRIGLGDYLIAATADVQDLQLATLNVWHFPMFEQLKPPFAVPGHRPRA

Download         Length: 402 bp

>T94 NC_000962:c1574513-1574112 [Mycobacterium tuberculosis H37Rv]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9443.58 Da        Isoelectric Point: 6.4631

>AT94 NP_215914.1 NC_000962:c1574767-1574510 [Mycobacterium tuberculosis H37Rv]
MKRTNIYLDEEQTASLDKLAAQEGVSRAELIRLLLNRALTTAGDDLASDLQAINDSFGTLRHLDPPVRRSGGREQHLAQV
WRATS

Download         Length: 258 bp

>AT94 NC_000962:c1574767-1574510 [Mycobacterium tuberculosis H37Rv]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T116 Mycobacterium tuberculosis H37Rv

42.276

98.4

0.416
T843 Bradyrhizobium diazoefficiens USDA 110

34.109

96.992

0.331

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P9WFA7

Antitoxin

Source ID Structure
AlphaFold DB P9WLZ1

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113] experimental


experimental experimental literature