Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2089210..2089712 Replicon chromosome
Accession NZ_CP026343
Organism Escherichia coli strain 9A

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C2R20_RS11095 Protein ID WP_000767829.1
Coordinates 2089210..2089464 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C2R20_RS11100 Protein ID WP_001259255.1
Coordinates 2089461..2089712 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2R20_RS11065 2084225..2084452 - 228 WP_000234896.1 sulfurtransferase TusA -
C2R20_RS11070 2084466..2085524 - 1059 WP_000492339.1 transport protein YeeE -
C2R20_RS11075 2085703..2087061 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C2R20_RS11080 2087051..2087113 - 63 WP_010723108.1 membrane protein YoeI -
C2R20_RS11085 2087328..2088257 - 930 WP_000803351.1 LysR family transcriptional regulator -
C2R20_RS11090 2088303..2089127 - 825 WP_000754737.1 SDR family oxidoreductase -
C2R20_RS11095 2089210..2089464 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C2R20_RS11100 2089461..2089712 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C2R20_RS11105 2089995..2090045 + 51 WP_001364200.1 his operon leader peptide -
C2R20_RS11115 2090191..2091090 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
C2R20_RS11120 2091096..2092400 + 1305 WP_000009594.1 histidinol dehydrogenase -
C2R20_RS11125 2092397..2093467 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
C2R20_RS11130 2093467..2094534 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T93914 WP_000767829.1 NZ_CP026343:c2089464-2089210 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T93914 NZ_CP026343:c2089464-2089210 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT93914 WP_001259255.1 NZ_CP026343:c2089712-2089461 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT93914 NZ_CP026343:c2089712-2089461 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References