Detailed information of TA system TA093806

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 119397..120034
• Replicon: plasmid unnamed
• Accession: NZ_CP026308
• Organism: Ralstonia solanacearum strain IBSBF 2571

Toxin (Protein)

• Gene name: higB
• Uniprot ID: F6G785
• Locus tag: C2124_RS16830
• Protein ID: WP_014618424.1
• Coordinates: 119397..119726 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2124_RS16835
• Protein ID: WP_003262799.1
• Coordinates: 119723..120034 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2124_RS16815	115106..116269	-	1164	WP_014618421.1	2-methylcitrate synthase	-
C2124_RS16820	116307..117203	-	897	WP_014618422.1	methylisocitrate lyase	-
C2124_RS16825	117397..119346	+	1950	WP_174265603.1	propionate catabolism operon regulatory protein PrpR	-
C2124_RS16830	119397..119726	+	330	WP_014618424.1	hypothetical protein	Toxin
C2124_RS16835	119723..120034	+	312	WP_003262799.1	transcriptional regulator	Antitoxin
C2124_RS16840	120185..120493	-	309	WP_003262797.1	type II toxin-antitoxin system YafQ family toxin	-
C2124_RS16845	120438..120749	-	312	WP_039551557.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
C2124_RS16850	121122..121328	+	207	WP_014618427.1	hypothetical protein	-
C2124_RS16855	121331..122164	-	834	WP_174265888.1	DUF2242 domain-containing protein	-
C2124_RS16860	122446..122817	+	372	WP_174265604.1	transcriptional repressor	-
C2124_RS16865	123010..123750	-	741	WP_003262792.1	metallophosphatase family protein	-
C2124_RS16870	123765..124079	+	315	WP_080773821.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY / cyaB / motA / cheB / cheY / adeG / acrB / fliI / fliM / flgG / flgC	1..1949116	1949116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12413.89 Da
• Isoelectric Point: 5.2566

>T93806 WP_014618424.1 NZ_CP026308:119397-119726 [Ralstonia solanacearum]
MDATPIELPGEHDLSDDEYSALQQELLAQPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGSSQFWMFVVYD
KDEAADLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 330 bp

>T93806 NZ_CP026308:119397-119726 [Ralstonia solanacearum]
ATGGACGCTACCCCCATTGAGTTGCCGGGGGAGCACGATCTCTCGGACGACGAGTACAGCGCCCTTCAACAGGAACTGCT
CGCCCAGCCGGACGCTGGCGACGTCATCAAGGGGACAGGTGGTCTACGCAAGCTGCGGTTTTCGGACAAGCGGCGCGGCA
AAGGTAAGCGCGGCGGTCTGCGCGTCATCTATTACCACTGGGATGGAAGCAGCCAATTCTGGATGTTTGTCGTCTACGAC
AAAGACGAGGCGGCCGATCTTTCCAGTGACGAGCGCAAAGTGCTGGCACGGCTGTTGGAGCAGGAAGTCAAGGCAAGGAG
TGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11620.59 Da
• Isoelectric Point: 10.3969

>AT93806 WP_003262799.1 NZ_CP026308:119723-120034 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVEVRVLQPIDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT93806 NZ_CP026308:119723-120034 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAATTGAAGGAGGGGATGGACGCGCTGGCCGCAGAGCGCGAAGGAAAGATGACCCTCCG
CAAGGTCGAGGTCCGCGTGCTCCAGCCGATTGATGTCACCGCCGAGGAAATCAAGGCCGTTCGCGCAGCGGCCCATGCCT
CACAGGCTGTCATGGCACGTCGGTTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGCACCGCCAAACCGAAC
ACCCAGGCGGCCGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: F6G785