Detailed information of TA system TA093797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 21040..21764
• Replicon: plasmid pKOR-ab4d
• Accession: NZ_CP026276
• Organism: Klebsiella oxytoca strain KONIH5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: C2U46_RS30340
• Protein ID: WP_023292165.1
• Coordinates: 21453..21764 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U46_RS30335
• Protein ID: WP_020801939.1
• Coordinates: 21040..21456 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U46_RS30290	16386..16694	-	309	WP_071599372.1	hypothetical protein	-
C2U46_RS30295	16754..17575	-	822	WP_020804822.1	DUF945 domain-containing protein	-
C2U46_RS30305	18401..19234	-	834	WP_020804845.1	type I restriction-modification system subunit M	-
C2U46_RS30310	19283..19429	-	147	WP_004152750.1	hypothetical protein	-
C2U46_RS30315	19524..19871	-	348	WP_020804846.1	hypothetical protein	-
C2U46_RS30320	19938..20318	-	381	WP_020802391.1	hypothetical protein	-
C2U46_RS30335	21040..21456	-	417	WP_020801939.1	helix-turn-helix domain-containing protein	Antitoxin
C2U46_RS30340	21453..21764	-	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
C2U46_RS30345	21871..22095	-	225	WP_042922612.1	hypothetical protein	-
C2U46_RS30350	22106..22312	-	207	WP_042922554.1	hypothetical protein	-
C2U46_RS30365	22949..23299	-	351	WP_004152758.1	hypothetical protein	-
C2U46_RS32650	23296..23568	-	273	WP_042922555.1	hypothetical protein	-
C2U46_RS30375	23926..24648	-	723	WP_020804573.1	plasmid SOS inhibition protein A	-
C2U46_RS30380	24645..25076	-	432	WP_020804574.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..282476	282476

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T93797 WP_023292165.1 NZ_CP026276:c21764-21453 [Klebsiella oxytoca]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T93797 NZ_CP026276:c21764-21453 [Klebsiella oxytoca]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCGAGACACTATCCGAATGATGCTGCGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGTGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15380.49 Da
• Isoelectric Point: 4.5542

>AT93797 WP_020801939.1 NZ_CP026276:c21456-21040 [Klebsiella oxytoca]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVG

Nucleotide

• Length: 417 bp

>AT93797 NZ_CP026276:c21456-21040 [Klebsiella oxytoca]
ATGATTTTTAGCGATGCCATTAAAGCGGCGAATGACCTGGCGAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCGCTTAAACTCGTTGAATATCTTCTGGAACATGACCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATGCCTGGGAAGATAACGCGGTGGAGTTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTA
TCGTTGCTGCGGGTTCTTATGCAGCAACACGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTTGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3