Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2159766..2160416 Replicon chromosome
Accession NZ_CP026275
Organism Klebsiella oxytoca strain KONIH5

Toxin (Protein)


Gene name higB Uniprot ID A0A0H3H3K0
Locus tag C2U46_RS10590 Protein ID WP_014227314.1
Coordinates 2160075..2160416 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H3H3Q5
Locus tag C2U46_RS10585 Protein ID WP_014227313.1
Coordinates 2159766..2160065 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U46_RS10565 2155651..2156370 - 720 WP_032694297.1 phosphonate utilization transcriptional regulator PhnR -
C2U46_RS10570 2156511..2157614 + 1104 WP_014227310.1 2-aminoethylphosphonate--pyruvate transaminase -
C2U46_RS10575 2157624..2158433 + 810 WP_004855618.1 phosphonoacetaldehyde hydrolase -
C2U46_RS10580 2158466..2159647 - 1182 WP_014227312.1 purine ribonucleoside efflux pump NepI -
C2U46_RS10585 2159766..2160065 - 300 WP_014227313.1 helix-turn-helix transcriptional regulator Antitoxin
C2U46_RS10590 2160075..2160416 - 342 WP_014227314.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C2U46_RS10595 2160667..2161119 - 453 WP_009653860.1 DUF1198 domain-containing protein -
C2U46_RS10600 2161297..2162688 - 1392 WP_004126843.1 hexose-6-phosphate:phosphate antiporter -
C2U46_RS10605 2162834..2164153 - 1320 WP_032694296.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13110.00 Da        Isoelectric Point: 5.7545

>T93785 WP_014227314.1 NZ_CP026275:c2160416-2160075 [Klebsiella oxytoca]
MWDVETTEVFDKWFEAQTEALKEDMLAAMVILSEYGPRLGRPFADTVNDSAFSNMKELRIQHQGRPIRAFFVFDPSRCGI
VLCAGDKTGLNEKRFYKDMIKLADAEYRKHLNQ

Download         Length: 342 bp

>T93785 NZ_CP026275:c2160416-2160075 [Klebsiella oxytoca]
ATGTGGGATGTTGAAACAACGGAGGTTTTTGACAAGTGGTTTGAGGCGCAGACCGAAGCGCTCAAAGAGGATATGCTCGC
CGCAATGGTGATCTTATCCGAGTATGGCCCCCGGTTGGGAAGGCCGTTTGCGGATACAGTAAACGATTCTGCCTTTTCGA
ATATGAAAGAGCTGCGCATTCAGCATCAAGGTAGACCCATCCGAGCTTTCTTTGTATTTGACCCTTCTCGTTGTGGCATC
GTGTTATGCGCCGGAGATAAAACAGGGTTAAATGAAAAGAGATTCTATAAAGATATGATCAAGCTTGCAGATGCTGAGTA
CCGTAAGCACCTTAATCAGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10966.87 Da        Isoelectric Point: 9.7256

>AT93785 WP_014227313.1 NZ_CP026275:c2160065-2159766 [Klebsiella oxytoca]
MATLKELMAKQSPESQARIAEKVEEMRQAIALSMLREELNMSQAELAKAMGVKQPTIAKMEQADNDPRLSTLKRYVAALG
GELSIDVKLPTGKRIAFHL

Download         Length: 300 bp

>AT93785 NZ_CP026275:c2160065-2159766 [Klebsiella oxytoca]
ATGGCGACCCTTAAAGAATTAATGGCCAAACAGAGCCCGGAAAGCCAGGCGCGTATTGCTGAAAAAGTCGAGGAGATGCG
CCAGGCTATCGCTTTAAGTATGTTAAGGGAAGAACTCAATATGTCTCAGGCAGAGCTGGCTAAGGCAATGGGCGTTAAAC
AACCCACCATAGCAAAAATGGAACAGGCTGATAACGACCCGCGTTTGTCAACGCTGAAGCGCTATGTTGCAGCGCTCGGG
GGGGAACTCAGTATTGATGTTAAGCTGCCAACCGGTAAGCGAATCGCATTCCATCTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H3K0

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3Q5

References