Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HigB-HigA |
| Location | 208035..208762 | Replicon | plasmid pCFR-9161 |
| Accession | NZ_CP026237 | ||
| Organism | Citrobacter freundii complex sp. CFNIH3 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A663AUB1 |
| Locus tag | C2U38_RS28255 | Protein ID | WP_000558568.1 |
| Coordinates | 208035..208346 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | V0AHC4 |
| Locus tag | C2U38_RS28260 | Protein ID | WP_000990392.1 |
| Coordinates | 208343..208762 (+) | Length | 140 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| C2U38_RS28210 | 203800..204768 | - | 969 | WP_088765930.1 | IS5 family transposase | - |
| C2U38_RS28220 | 204790..205011 | - | 222 | WP_016237028.1 | hypothetical protein | - |
| C2U38_RS28225 | 205025..205228 | - | 204 | WP_004213596.1 | hemolysin expression modulator Hha | - |
| C2U38_RS28230 | 205289..205780 | - | 492 | WP_004206662.1 | hypothetical protein | - |
| C2U38_RS28240 | 206164..206868 | - | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| C2U38_RS28245 | 206933..207394 | + | 462 | Protein_201 | lactate utilization protein C | - |
| C2U38_RS28250 | 207444..207824 | - | 381 | Protein_202 | gluconate permease | - |
| C2U38_RS28255 | 208035..208346 | + | 312 | WP_000558568.1 | type II toxin-antitoxin system HigB family toxin | Toxin |
| C2U38_RS28260 | 208343..208762 | + | 420 | WP_000990392.1 | helix-turn-helix domain-containing protein | Antitoxin |
| C2U38_RS28270 | 210095..210406 | - | 312 | WP_001324897.1 | hypothetical protein | - |
| C2U38_RS28275 | 210568..210918 | + | 351 | WP_000928911.1 | metalloregulator ArsR/SmtB family transcription factor | - |
| C2U38_RS28280 | 211223..211699 | - | 477 | WP_000043177.1 | DNA starvation/stationary phase protection protein | - |
| C2U38_RS28285 | 211823..212770 | - | 948 | Protein_209 | transposase | - |
| C2U38_RS28290 | 212836..213540 | + | 705 | WP_088765929.1 | IS6 family transposase | - |
| C2U38_RS28295 | 213594..213659 | + | 66 | Protein_211 | cellulose synthase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | IScluster/Tn | - | - | 193291..215433 | 22142 | |
| - | inside | Mobilizable plasmid | - | - | 1..235027 | 235027 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12372.11 Da Isoelectric Point: 10.0935
>T93751 WP_000558568.1 NZ_CP026237:208035-208346 [Citrobacter freundii complex sp. CFNIH3]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE
Download Length: 312 bp
>T93751 NZ_CP026237:208035-208346 [Citrobacter freundii complex sp. CFNIH3]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA
Antitoxin
Download Length: 140 a.a. Molecular weight: 15534.71 Da Isoelectric Point: 4.4702
>AT93751 WP_000990392.1 NZ_CP026237:208343-208762 [Citrobacter freundii complex sp. CFNIH3]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA
Download Length: 420 bp
>AT93751 NZ_CP026237:208343-208762 [Citrobacter freundii complex sp. CFNIH3]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTTCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTTCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A663AUB1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | V0AHC4 |