Detailed information of TA system TA093733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1916793..1917453
• Replicon: chromosome
• Accession: NZ_CP026235
• Organism: Citrobacter freundii complex sp. CFNIH3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2I8S535
• Locus tag: C2U38_RS09600
• Protein ID: WP_053390179.1
• Coordinates: 1916793..1917146 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U38_RS09605
• Protein ID: WP_103283718.1
• Coordinates: 1917151..1917453 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U38_RS28610	1913009..1913611	+	603	WP_199773185.1	hypothetical protein	-
C2U38_RS09585	1913624..1915228	+	1605	WP_103283715.1	hypothetical protein	-
C2U38_RS09590	1915609..1916076	+	468	WP_103283716.1	hypothetical protein	-
C2U38_RS09595	1916073..1916294	+	222	WP_103283717.1	helix-turn-helix domain-containing protein	-
C2U38_RS09600	1916793..1917146	+	354	WP_053390179.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U38_RS09605	1917151..1917453	+	303	WP_103283718.1	XRE family transcriptional regulator	Antitoxin
C2U38_RS09610	1917520..1917822	-	303	WP_146053242.1	hypothetical protein	-
C2U38_RS09615	1917954..1918841	+	888	WP_103283719.1	integrase domain-containing protein	-
C2U38_RS09620	1919523..1920785	-	1263	WP_103283720.1	integrase arm-type DNA-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1908949..1917453	8504

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13442.36 Da
• Isoelectric Point: 9.0134

>T93733 WP_053390179.1 NZ_CP026235:1916793-1917146 [Citrobacter freundii complex sp. CFNIH3]
VWTIKTTDMFDHWFSSLNDIDRASVLAALLVLREKGPGLSRPYADTLRGSRFSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMIPVADREFTNWLNTIKEKE

Nucleotide

• Length: 354 bp

>T93733 NZ_CP026235:1916793-1917146 [Citrobacter freundii complex sp. CFNIH3]
GTGTGGACGATTAAAACAACGGATATGTTTGATCACTGGTTTTCCTCGCTCAATGATATCGATCGGGCCAGCGTGCTTGC
AGCGCTGTTGGTCTTGCGGGAAAAGGGGCCAGGTTTGTCCAGACCTTATGCCGATACGCTTAGAGGCTCCCGCTTTAGCA
ACATGAAAGAGTTGCGCATTCAGAGCCGGGGCGATCCGATACGGGCATTTTTCGCTTTTGATCCAGCCCGTACCGGCATT
GTACTTTGTGCCGGAAATAAGGTCGGCAATGAAAAACGTTTTTACGATGAGATGATCCCGGTTGCAGATCGGGAATTCAC
GAACTGGCTGAACACAATAAAAGAGAAGGAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11054.88 Da
• Isoelectric Point: 6.4738

>AT93733 WP_103283718.1 NZ_CP026235:1917151-1917453 [Citrobacter freundii complex sp. CFNIH3]
MGRTLEQLIADEKPEVVAEAQAMATDILLNIHLAELREKVQKTQVEMAKALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDIELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT93733 NZ_CP026235:1917151-1917453 [Citrobacter freundii complex sp. CFNIH3]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAAAAACCTGAAGTCGTGGCCGAAGCTCAGGCGATGGCGACGGACAT
CCTGCTCAACATTCACCTTGCCGAACTGCGCGAGAAAGTGCAGAAAACGCAGGTCGAAATGGCGAAGGCTCTGGGGATCA
GGCAACCTACCGTTGCAGGAATGGAAAAACCGGGGCGCGACCTGAAGCTTTCCACGCTGAAACGCTACGTTGAAGCGACC
GGCGGCAAGCTACGCCTTGATATTGAACTGCCGGATGGTTCTCACTATGGATTTGTACTATAA

Structures

Toxin

• AlphaFold DB: A0A2I8S535