Detailed information of TA system TA093721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 25055..25712
• Replicon: plasmid pKPC-8e09
• Accession: NZ_CP026225
• Organism: Aeromonas sp. ASNIH3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: C2U39_RS23160
• Protein ID: WP_000270043.1
• Coordinates: 25362..25712 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U39_RS23155
• Protein ID: WP_000124640.1
• Coordinates: 25055..25357 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U39_RS23105	20680..21108	+	429	WP_000591074.1	hypothetical protein	-
C2U39_RS23110	21165..21524	+	360	WP_000422768.1	hypothetical protein	-
C2U39_RS23115	21524..21970	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
C2U39_RS23120	21967..22485	+	519	WP_000210756.1	nitrite reductase	-
C2U39_RS23125	22485..22715	+	231	WP_000972663.1	hypothetical protein	-
C2U39_RS23130	22702..23559	+	858	WP_001167032.1	hypothetical protein	-
C2U39_RS23140	23790..24317	+	528	WP_001236377.1	thermonuclease family protein	-
C2U39_RS23145	24375..24647	+	273	WP_001043047.1	HU family DNA-binding protein	-
C2U39_RS23150	24735..25028	+	294	WP_001239997.1	chromosome segregation protein ParM	-
C2U39_RS23155	25055..25357	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
C2U39_RS23160	25362..25712	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2U39_RS23165	25875..26423	+	549	WP_001061574.1	transcriptional regulator	-
C2U39_RS23175	26764..26958	+	195	WP_000343597.1	hypothetical protein	-
C2U39_RS23180	26969..27340	+	372	WP_000516916.1	hypothetical protein	-
C2U39_RS23185	27333..27803	+	471	WP_001281821.1	hypothetical protein	-
C2U39_RS23190	27818..28153	-	336	WP_000683476.1	hypothetical protein	-
C2U39_RS23195	28250..28738	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
C2U39_RS23200	28741..29238	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaFOX-5 / blaCARB-2 / aadA2 / cmlA1 / qacE / sul1 / qnrA1 / msr(E) / mph(E) / blaKPC-2	-	1..178635	178635

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T93721 WP_000270043.1 NZ_CP026225:c25712-25362 [Aeromonas sp. ASNIH3]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T93721 NZ_CP026225:c25712-25362 [Aeromonas sp. ASNIH3]
ATGTGGGTCATCGAGACAACCGACACCTTTGATGAGTGGTTCGATGCTCTGGATGATACCGATAGAGCAAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGCCCCATGCTGTCGAGGCCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTTCGGGTCCAAAGCAAAGGAGATCCTATCAGAGCGTTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGTGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT93721 WP_000124640.1 NZ_CP026225:c25357-25055 [Aeromonas sp. ASNIH3]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT93721 NZ_CP026225:c25357-25055 [Aeromonas sp. ASNIH3]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9