Detailed information of TA system TA093673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 166161..166885
• Replicon: plasmid pKPC-224e
• Accession: NZ_CP026179
• Organism: Klebsiella pneumoniae strain KPNIH49

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2J4YLV3
• Locus tag: C2U49_RS28680
• Protein ID: WP_023292165.1
• Coordinates: 166574..166885 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C2U49_RS28675
• Protein ID: WP_020801939.1
• Coordinates: 166161..166577 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2U49_RS28630	161507..161815	-	309	WP_071599372.1	hypothetical protein	-
C2U49_RS28635	161875..162696	-	822	WP_020804822.1	DUF945 domain-containing protein	-
C2U49_RS28645	163522..164355	-	834	WP_020804845.1	type I restriction-modification system subunit M	-
C2U49_RS28650	164404..164550	-	147	WP_004152750.1	hypothetical protein	-
C2U49_RS28655	164645..164992	-	348	WP_020804846.1	hypothetical protein	-
C2U49_RS28660	165059..165439	-	381	WP_020802391.1	hypothetical protein	-
C2U49_RS28675	166161..166577	-	417	WP_020801939.1	helix-turn-helix domain-containing protein	Antitoxin
C2U49_RS28680	166574..166885	-	312	WP_023292165.1	type II toxin-antitoxin system HigB family toxin	Toxin
C2U49_RS28685	166992..167216	-	225	WP_042922612.1	hypothetical protein	-
C2U49_RS28690	167227..167433	-	207	WP_042922554.1	hypothetical protein	-
C2U49_RS28705	168070..168420	-	351	WP_004152758.1	hypothetical protein	-
C2U49_RS34470	168417..168689	-	273	WP_042922555.1	hypothetical protein	-
C2U49_RS28715	169047..169769	-	723	WP_020804573.1	plasmid SOS inhibition protein A	-
C2U49_RS28720	169766..170197	-	432	WP_020804574.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / blaKPC-2 / qnrB19 / blaSHV-12	-	1..237571	237571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-133)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12497.32 Da
• Isoelectric Point: 9.9242

>T93673 WP_023292165.1 NZ_CP026179:c166885-166574 [Klebsiella pneumoniae]
VHVISRAPFDEAARHYPNDAAAIDDTYRVLKRVVAKKPDELKRYFTSLDRMKYREKWWVIDIGGNNLRMMFFADFERGKI
FVKHITTHAEYDRLTKYYREHKE

Nucleotide

• Length: 312 bp

>T93673 NZ_CP026179:c166885-166574 [Klebsiella pneumoniae]
GTGCATGTTATATCACGAGCCCCTTTTGATGAGGCGGCGAGACACTATCCGAATGATGCTGCGGCCATCGATGACACCTA
TCGTGTACTAAAGCGGGTTGTCGCAAAAAAACCGGATGAGCTAAAGCGATACTTTACTAGTCTGGATCGCATGAAATACC
GGGAAAAATGGTGGGTTATCGATATCGGTGGTAATAACCTTAGAATGATGTTTTTTGCTGATTTTGAGCGCGGAAAGATA
TTCGTCAAACACATAACCACCCATGCTGAATATGATCGCTTAACGAAATATTATCGGGAGCACAAAGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15380.49 Da
• Isoelectric Point: 4.5542

>AT93673 WP_020801939.1 NZ_CP026179:c166577-166161 [Klebsiella pneumoniae]
MIFSDAIKAANDLASIVPLLGGSSSRKDYEEALKLVEYLLEHDPDSPLVDMLTARIDAWEDNAVEFEEFNTRIEAGKNGV
SLLRVLMQQHGLSQSDFENEIGKKSLVSRILSGERSLTLDHMRALANRFQIPVSMFVG

Nucleotide

• Length: 417 bp

>AT93673 NZ_CP026179:c166577-166161 [Klebsiella pneumoniae]
ATGATTTTTAGCGATGCCATTAAAGCGGCGAATGACCTGGCGAGTATTGTCCCTCTGCTGGGTGGCAGCTCGTCCCGCAA
AGATTACGAAGAGGCGCTTAAACTCGTTGAATATCTTCTGGAACATGACCCGGACAGCCCGCTGGTTGATATGCTGACTG
CCCGCATCGATGCCTGGGAAGATAACGCGGTGGAGTTTGAAGAATTTAATACGCGCATCGAAGCAGGCAAAAACGGTGTA
TCGTTGCTGCGGGTTCTTATGCAGCAACACGGGCTTTCACAGTCCGATTTTGAAAACGAGATAGGCAAAAAATCGCTCGT
CAGCCGGATCCTCAGTGGTGAACGCAGTCTGACCCTTGATCACATGCGGGCGCTGGCAAATCGATTTCAAATCCCGGTCT
CGATGTTTGTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A2J4YLV3