93645

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3834510..3835107 Replicon chromosome
Accession NZ_CP026177
Organism Klebsiella pneumoniae strain KPNIH50

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag C2U50_RS23590 Protein ID WP_004142563.1
Coordinates 3834510..3834827 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag C2U50_RS23595 Protein ID WP_004142561.1
Coordinates 3834820..3835107 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2U50_RS23570 3830210..3831637 - 1428 WP_040236578.1 MFS transporter -
C2U50_RS23575 3831746..3832612 + 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
C2U50_RS23585 3833282..3834325 + 1044 WP_040236579.1 DUF2157 domain-containing protein -
C2U50_RS23590 3834510..3834827 + 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C2U50_RS23595 3834820..3835107 + 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
C2U50_RS23600 3835372..3836025 - 654 WP_004176973.1 oxygen-insensitive NAD(P)H nitroreductase -
C2U50_RS23605 3836145..3836513 - 369 WP_040236580.1 MmcQ/YjbR family DNA-binding protein -
C2U50_RS23610 3836513..3837094 - 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
C2U50_RS23615 3837274..3838389 + 1116 WP_012737592.1 MBL fold metallo-hydrolase -
C2U50_RS23620 3838420..3838761 + 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
C2U50_RS23625 3838779..3839027 - 249 WP_002893037.1 DUF1158 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T93645 WP_004142563.1 NZ_CP026177:3834510-3834827 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T93645 NZ_CP026177:3834510-3834827 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT93645 WP_004142561.1 NZ_CP026177:3834820-3835107 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT93645 NZ_CP026177:3834820-3835107 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACGGTCTCCAGAATGGAAGCCAATATCACCCGGGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References