Detailed information of TA system TA093546

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 86662..87088
• Replicon: plasmid pF93-2_1
• Accession: NZ_CP026158
• Organism: Klebsiella pneumoniae strain F93-2

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C2M12_RS26895
• Protein ID: WP_001312861.1
• Coordinates: 86662..86820 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	86864..87088 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2M12_RS25800	81685..81912	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
C2M12_RS25805	82049..82720	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
C2M12_RS25810	82914..83297	-	384	WP_001354030.1	relaxosome protein TraM	-
C2M12_RS25820	83620..84222	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
C2M12_RS25825	84519..85340	-	822	WP_001234445.1	DUF945 domain-containing protein	-
C2M12_RS25830	85451..85747	-	297	WP_001272251.1	hypothetical protein	-
C2M12_RS27005	86047..86424	+	378	Protein_102	hypothetical protein	-
C2M12_RS26895	86662..86820	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	86864..87088	-	225	NuclAT_0	-	Antitoxin
-	86864..87088	-	225	NuclAT_0	-	Antitoxin
-	86864..87088	-	225	NuclAT_0	-	Antitoxin
-	86864..87088	-	225	NuclAT_0	-	Antitoxin
C2M12_RS25855	87100..87819	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
C2M12_RS25860	87816..88250	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C2M12_RS25865	88319..90342	-	2024	Protein_106	ParB/RepB/Spo0J family partition protein	-
C2M12_RS25870	90403..90636	-	234	WP_000006003.1	DUF905 family protein	-
C2M12_RS25875	90694..91221	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / qnrS1 / aac(3)-IIa / mph(A) / sul1 / qacE / aadA5	-	1..95560	95560

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T93546 WP_001312861.1 NZ_CP026158:c86820-86662 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T93546 NZ_CP026158:c86820-86662 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT93546 NZ_CP026158:c87088-86864 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin